TY - JOUR
T1 - Brain cell reservoirs of latent virus in presymptomatic HIV-infected individuals
AU - Thompson, Katherine A.
AU - Cherry, Catherine L.
AU - Bell, Jeanne E
AU - McLean, Catriona A.
PY - 2011/10
Y1 - 2011/10
N2 - We detected HIV-1 DNA in pure populations of perivascular macrophages, parenchymal microglia, and astrocytes, isolated using laser microdissection from brain tissue of five untreated individuals who died in the presymptomatic stage of infection from non-HIV causes. HIV-1 DNA was detected in the three cell populations, most consistently in perivascular macrophages, without evidence of productive infection. The percentage of PCR reactions detecting HIV-1 DNA in perivascular macrophages correlated inversely with peripheral blood CD4 counts. These findings demonstrate that brain cell reservoirs of latent HIV-1 exist before pathological HIV encephalitis and suggest that perivascular macrophage trafficking of latent virus into the brain increases with immunosuppression.
AB - We detected HIV-1 DNA in pure populations of perivascular macrophages, parenchymal microglia, and astrocytes, isolated using laser microdissection from brain tissue of five untreated individuals who died in the presymptomatic stage of infection from non-HIV causes. HIV-1 DNA was detected in the three cell populations, most consistently in perivascular macrophages, without evidence of productive infection. The percentage of PCR reactions detecting HIV-1 DNA in perivascular macrophages correlated inversely with peripheral blood CD4 counts. These findings demonstrate that brain cell reservoirs of latent HIV-1 exist before pathological HIV encephalitis and suggest that perivascular macrophage trafficking of latent virus into the brain increases with immunosuppression.
UR - http://www.scopus.com/inward/record.url?scp=80053278411&partnerID=8YFLogxK
U2 - 10.1016/j.ajpath.2011.06.039
DO - 10.1016/j.ajpath.2011.06.039
M3 - Article
AN - SCOPUS:80053278411
SN - 0002-9440
VL - 179
SP - 1623
EP - 1629
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 4
ER -