Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing

K. N. Harikrishnan, Maggie Z. Chow, Emma K. Baker, Sharmistha Pal, Sahar Bassal, Daniella Brasacchio, Li Wang, Jeff M. Craig, Peter L. Jones, Saïd Sif, Assam El-Osta

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.

Original languageEnglish
Pages (from-to)254-264
Number of pages11
JournalNature Genetics
Volume37
Issue number3
DOIs
Publication statusPublished - 2005
Externally publishedYes

Cite this

Harikrishnan, K. N. ; Chow, Maggie Z. ; Baker, Emma K. ; Pal, Sharmistha ; Bassal, Sahar ; Brasacchio, Daniella ; Wang, Li ; Craig, Jeff M. ; Jones, Peter L. ; Sif, Saïd ; El-Osta, Assam. / Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing. In: Nature Genetics. 2005 ; Vol. 37, No. 3. pp. 254-264.
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abstract = "Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.",
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Harikrishnan, KN, Chow, MZ, Baker, EK, Pal, S, Bassal, S, Brasacchio, D, Wang, L, Craig, JM, Jones, PL, Sif, S & El-Osta, A 2005, 'Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing' Nature Genetics, vol. 37, no. 3, pp. 254-264. https://doi.org/10.1038/ng1516

Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing. / Harikrishnan, K. N.; Chow, Maggie Z.; Baker, Emma K.; Pal, Sharmistha; Bassal, Sahar; Brasacchio, Daniella; Wang, Li; Craig, Jeff M.; Jones, Peter L.; Sif, Saïd; El-Osta, Assam.

In: Nature Genetics, Vol. 37, No. 3, 2005, p. 254-264.

Research output: Contribution to journalArticleResearchpeer-review

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