Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing

K. N. Harikrishnan; Maggie Z. Chow; Emma K. Baker; Sharmistha Pal; Sahar Bassal; Daniella Brasacchio; Li Wang; Jeff M. Craig; Peter L. Jones; Saïd Sif; Assam El-Osta

doi:10.1038/ng1516

Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing

K. N. Harikrishnan, Maggie Z. Chow, Emma K. Baker, Sharmistha Pal, Sahar Bassal, Daniella Brasacchio, Li Wang, Jeff M. Craig, Peter L. Jones, Saïd Sif, Assam El-Osta

Research output: Contribution to journal › Article › Research › peer-review

225 Citations (Scopus)

Abstract

Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.

Original language	English
Pages (from-to)	254-264
Number of pages	11
Journal	Nature Genetics
Volume	37
Issue number	3
DOIs	https://doi.org/10.1038/ng1516
Publication status	Published - 2005
Externally published	Yes

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10.1038/ng1516

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Cite this

@article{5e1463f44d3c4bb1882bba9ece034551,

title = "Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing",

abstract = "Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.",

author = "Harikrishnan, {K. N.} and Chow, {Maggie Z.} and Baker, {Emma K.} and Sharmistha Pal and Sahar Bassal and Daniella Brasacchio and Li Wang and Craig, {Jeff M.} and Jones, {Peter L.} and Sa{\"i}d Sif and Assam El-Osta",

year = "2005",

doi = "10.1038/ng1516",

language = "English",

volume = "37",

pages = "254--264",

journal = "Nature Genetics",

issn = "1061-4036",

publisher = "Nature Publishing Group",

number = "3",

}

Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing. / Harikrishnan, K. N.; Chow, Maggie Z.; Baker, Emma K.; Pal, Sharmistha; Bassal, Sahar; Brasacchio, Daniella; Wang, Li; Craig, Jeff M.; Jones, Peter L.; Sif, Saïd; El-Osta, Assam.

In: Nature Genetics, Vol. 37, No. 3, 2005, p. 254-264.

Research output: Contribution to journal › Article › Research › peer-review

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T1 - Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing

AU - Harikrishnan, K. N.

AU - Chow, Maggie Z.

AU - Baker, Emma K.

AU - Pal, Sharmistha

AU - Bassal, Sahar

AU - Brasacchio, Daniella

AU - Wang, Li

AU - Craig, Jeff M.

AU - Jones, Peter L.

AU - Sif, Saïd

AU - El-Osta, Assam

PY - 2005

Y1 - 2005

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AB - Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.

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