Abstract
Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.
| Original language | English |
|---|---|
| Pages (from-to) | 254-264 |
| Number of pages | 11 |
| Journal | Nature Genetics |
| Volume | 37 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2005 |
| Externally published | Yes |
Cite this
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Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing. / Harikrishnan, K. N.; Chow, Maggie Z.; Baker, Emma K.; Pal, Sharmistha; Bassal, Sahar; Brasacchio, Daniella; Wang, Li; Craig, Jeff M.; Jones, Peter L.; Sif, Saïd; El-Osta, Assam.
In: Nature Genetics, Vol. 37, No. 3, 2005, p. 254-264.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing
AU - Harikrishnan, K. N.
AU - Chow, Maggie Z.
AU - Baker, Emma K.
AU - Pal, Sharmistha
AU - Bassal, Sahar
AU - Brasacchio, Daniella
AU - Wang, Li
AU - Craig, Jeff M.
AU - Jones, Peter L.
AU - Sif, Saïd
AU - El-Osta, Assam
PY - 2005
Y1 - 2005
N2 - Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.
AB - Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.
UR - http://www.scopus.com/inward/record.url?scp=20144379888&partnerID=8YFLogxK
U2 - 10.1038/ng1516
DO - 10.1038/ng1516
M3 - Article
C2 - 15696166
AN - SCOPUS:20144379888
VL - 37
SP - 254
EP - 264
JO - Nature Genetics
JF - Nature Genetics
SN - 1061-4036
IS - 3
ER -