Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing

K. N. Harikrishnan, Maggie Z. Chow, Emma K. Baker, Sharmistha Pal, Sahar Bassal, Daniella Brasacchio, Li Wang, Jeff M. Craig, Peter L. Jones, Saïd Sif, Assam El-Osta

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225 Citations (Scopus)

Abstract

Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.

Original languageEnglish
Pages (from-to)254-264
Number of pages11
JournalNature Genetics
Volume37
Issue number3
DOIs
Publication statusPublished - 2005
Externally publishedYes

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