Bone marrow transplantation and RNAseq analysis of Gpr21−/− monocytes reveals reduced migratory function and downregulation of inflammatory genes

Research output: Contribution to conferenceAbstractOther

Abstract

Immune cell infiltration into tissues produces chronic low-grade inflammation leading to obesity-induced insulin resistance . Insight into this mechanistic link has revealed activation of various receptors including the orphan G protein-coupled receptor, GPR21 .GPR21 is widely expressed throughout the body, and on immune cells including monocytes and macrophages . Transplantation of bone marrow from wild-type or Gpr21 animals into irradiated wild-type animals fed normal chow (13.5% fat), or high fat diet (HFD; 60% fat) for 27 weeks, revealed no improvement in glucose homeostasis, but an improvement in insulin sensitivity and a decrease in immune cell infiltration into eWAT. Furthermore, a decrease in the migratory ability of isolated CD11b bone marrow monocytes from Gpr21 mice compared to wild-type in response to monocyte chemo attractant protein-1 (MCP-1) was observed. These data were confirmed using aPKH26 monocyte tracking study. RNAseq analysis of CD11b monocytes from Gpr21 mice revealed an overall significant effect on genes involved in inflammation and cell migration, including Il6, Ccl2, Cxcl2 and members of the TLR family, supporting the reduced functional response. Significant changes in genes involved in atherosclerosis was also observed, including Apoe and Nr4a1. These data indicate that GPR21 is involved in the chemotaxis of specific immune cells. Targeting this receptor may prove beneficial for the treatment of T2DM and its complications.
Original languageEnglish
Number of pages2
DOIs
Publication statusPublished - 2019
EventAustralia and New Zealand Obesity Society Annual Meeting 2018 - Melbourne Convention & Exhibition Centre, Melbourne, Australia
Duration: 16 Oct 201818 Oct 2018
https://web.archive.org/web/20180912131044/http://www.anzos-mdo2018.org/

Conference

ConferenceAustralia and New Zealand Obesity Society Annual Meeting 2018
Abbreviated titleANZOS 2018
CountryAustralia
CityMelbourne
Period16/10/1818/10/18
OtherWelcome to the Joint Scientific Meeting of The Australian and New Zealand Obesity Society (ANZOS) and the Breakthrough Discoveries in Metabolism, Diabetes and Obesity.

With great enthusiasm we are pleased to invite you to attend the 2018 Joint Scientific Meeting which will be held at the Melbourne Exhibition and Convention Centre from the 16th - 18th October 2018.

This conference will feature a diverse and vibrant group of established international and national experts. You will have direct access to opinion-leaders, experts and key decision makers which will afford an invaluable opportunity to gain knowledge, foster relationships and create networks. We look forward to your active participation and contribution.
Internet address

Cite this

@conference{88f52bb3521c4c759e23ff3f5f918893,
title = "Bone marrow transplantation and RNAseq analysis of Gpr21−/− monocytes reveals reduced migratory function and downregulation of inflammatory genes",
abstract = "Immune cell infiltration into tissues produces chronic low-grade inflammation leading to obesity-induced insulin resistance . Insight into this mechanistic link has revealed activation of various receptors including the orphan G protein-coupled receptor, GPR21 .GPR21 is widely expressed throughout the body, and on immune cells including monocytes and macrophages . Transplantation of bone marrow from wild-type or Gpr21 animals into irradiated wild-type animals fed normal chow (13.5{\%} fat), or high fat diet (HFD; 60{\%} fat) for 27 weeks, revealed no improvement in glucose homeostasis, but an improvement in insulin sensitivity and a decrease in immune cell infiltration into eWAT. Furthermore, a decrease in the migratory ability of isolated CD11b bone marrow monocytes from Gpr21 mice compared to wild-type in response to monocyte chemo attractant protein-1 (MCP-1) was observed. These data were confirmed using aPKH26 monocyte tracking study. RNAseq analysis of CD11b monocytes from Gpr21 mice revealed an overall significant effect on genes involved in inflammation and cell migration, including Il6, Ccl2, Cxcl2 and members of the TLR family, supporting the reduced functional response. Significant changes in genes involved in atherosclerosis was also observed, including Apoe and Nr4a1. These data indicate that GPR21 is involved in the chemotaxis of specific immune cells. Targeting this receptor may prove beneficial for the treatment of T2DM and its complications.",
author = "Darren Riddy and Helene Kammoun and Sanja Bosnyak-Gladovic and Mark Ziemann and Roger Summers and Sexton, {Patrick Michael} and Murphy, {Andrew James M.} and Christopher Langmead",
year = "2019",
doi = "10.1016/j.orcp.2018.11.162",
language = "English",
note = "Australia and New Zealand Obesity Society Annual Meeting 2018, ANZOS 2018 ; Conference date: 16-10-2018 Through 18-10-2018",
url = "https://web.archive.org/web/20180912131044/http://www.anzos-mdo2018.org/",

}

Bone marrow transplantation and RNAseq analysis of Gpr21−/− monocytes reveals reduced migratory function and downregulation of inflammatory genes. / Riddy, Darren; Kammoun, Helene; Bosnyak-Gladovic, Sanja; Ziemann, Mark; Summers, Roger; Sexton, Patrick Michael; Murphy, Andrew James M.; Langmead, Christopher.

2019. Abstract from Australia and New Zealand Obesity Society Annual Meeting 2018, Melbourne, Australia.

Research output: Contribution to conferenceAbstractOther

TY - CONF

T1 - Bone marrow transplantation and RNAseq analysis of Gpr21−/− monocytes reveals reduced migratory function and downregulation of inflammatory genes

AU - Riddy, Darren

AU - Kammoun, Helene

AU - Bosnyak-Gladovic, Sanja

AU - Ziemann, Mark

AU - Summers, Roger

AU - Sexton, Patrick Michael

AU - Murphy, Andrew James M.

AU - Langmead, Christopher

PY - 2019

Y1 - 2019

N2 - Immune cell infiltration into tissues produces chronic low-grade inflammation leading to obesity-induced insulin resistance . Insight into this mechanistic link has revealed activation of various receptors including the orphan G protein-coupled receptor, GPR21 .GPR21 is widely expressed throughout the body, and on immune cells including monocytes and macrophages . Transplantation of bone marrow from wild-type or Gpr21 animals into irradiated wild-type animals fed normal chow (13.5% fat), or high fat diet (HFD; 60% fat) for 27 weeks, revealed no improvement in glucose homeostasis, but an improvement in insulin sensitivity and a decrease in immune cell infiltration into eWAT. Furthermore, a decrease in the migratory ability of isolated CD11b bone marrow monocytes from Gpr21 mice compared to wild-type in response to monocyte chemo attractant protein-1 (MCP-1) was observed. These data were confirmed using aPKH26 monocyte tracking study. RNAseq analysis of CD11b monocytes from Gpr21 mice revealed an overall significant effect on genes involved in inflammation and cell migration, including Il6, Ccl2, Cxcl2 and members of the TLR family, supporting the reduced functional response. Significant changes in genes involved in atherosclerosis was also observed, including Apoe and Nr4a1. These data indicate that GPR21 is involved in the chemotaxis of specific immune cells. Targeting this receptor may prove beneficial for the treatment of T2DM and its complications.

AB - Immune cell infiltration into tissues produces chronic low-grade inflammation leading to obesity-induced insulin resistance . Insight into this mechanistic link has revealed activation of various receptors including the orphan G protein-coupled receptor, GPR21 .GPR21 is widely expressed throughout the body, and on immune cells including monocytes and macrophages . Transplantation of bone marrow from wild-type or Gpr21 animals into irradiated wild-type animals fed normal chow (13.5% fat), or high fat diet (HFD; 60% fat) for 27 weeks, revealed no improvement in glucose homeostasis, but an improvement in insulin sensitivity and a decrease in immune cell infiltration into eWAT. Furthermore, a decrease in the migratory ability of isolated CD11b bone marrow monocytes from Gpr21 mice compared to wild-type in response to monocyte chemo attractant protein-1 (MCP-1) was observed. These data were confirmed using aPKH26 monocyte tracking study. RNAseq analysis of CD11b monocytes from Gpr21 mice revealed an overall significant effect on genes involved in inflammation and cell migration, including Il6, Ccl2, Cxcl2 and members of the TLR family, supporting the reduced functional response. Significant changes in genes involved in atherosclerosis was also observed, including Apoe and Nr4a1. These data indicate that GPR21 is involved in the chemotaxis of specific immune cells. Targeting this receptor may prove beneficial for the treatment of T2DM and its complications.

U2 - 10.1016/j.orcp.2018.11.162

DO - 10.1016/j.orcp.2018.11.162

M3 - Abstract

ER -