Blood CD9+ B cell, a biomarker of bronchiolitis obliterans syndrome after lung transplantation

Carole Brosseau, Richard Danger, Maxim Durand, Eugénie Durand, Aurore Foureau, Philippe Lacoste, Adrien Tissot, Antoine Roux, Martine Reynaud-Gaubert, Romain Kessler, Sacha Mussot, Claire Dromer, Olivier Brugière, Jean François Mornex, Romain Guillemain, Johanna Claustre, Antoine Magnan, Sophie Brouard, COLT and SysCLAD Consortia

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Bronchiolitis obliterans syndrome is the main limitation for long-term survival after lung transplantation. Some specific B cell populations are associated with long-term graft acceptance. We aimed to monitor the B cell profile during early development of bronchiolitis obliterans syndrome after lung transplantation. The B cell longitudinal profile was analyzed in peripheral blood mononuclear cells from patients with bronchiolitis obliterans syndrome and patients who remained stable over 3 years of follow-up. CD24hiCD38hi transitional B cells were increased in stable patients only, and reached a peak 24 months after transplantation, whereas they remained unchanged in patients who developed a bronchiolitis obliterans syndrome. These CD24hiCD38hi transitional B cells specifically secrete IL-10 and express CD9. Thus, patients with a total CD9+ B cell frequency below 6.6% displayed significantly higher incidence of bronchiolitis obliterans syndrome (AUC = 0.836, PPV = 0.75, NPV = 1). These data are the first to associate IL-10-secreting CD24hiCD38hi transitional B cells expressing CD9 with better allograft outcome in lung transplant recipients. CD9-expressing B cells appear as a contributor to a favorable environment essential for the maintenance of long-term stable graft function and as a new predictive biomarker of bronchiolitis obliterans syndrome–free survival.

Original languageEnglish
Pages (from-to)3162-3175
Number of pages14
JournalAmerican Journal of Transplantation
Volume19
Issue number11
DOIs
Publication statusPublished - 1 Nov 2019
Externally publishedYes

Keywords

  • B cell biology
  • basic (laboratory) research/science
  • biomarker
  • bronchiolitis obliterans (BOS)
  • lung transplantation/pulmonology
  • translational research/science

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