TY - JOUR
T1 - Blockage of NF-κB signaling by selective ablation of an mRNA target by 2-5A antisense chimeras
AU - Maran, Avudaiappan
AU - Maitra, Ratan K.
AU - Kumar, Aseem
AU - Dong, Beihua
AU - Xiao, Wei
AU - Li, Guiying
AU - Williams, Bryan R.G.
AU - Torrence, Paul F.
AU - Silverman, Robert H.
PY - 1994/8/5
Y1 - 1994/8/5
N2 - Activation of 2-5A-dependent ribonuclease by 5'-phosphorylated, 2',5'-linked oligoadenylates, known as 2-5A, is one pathway of interferon action. Unaided uptake into HeLa cells of 2-5A linked to an antisense oligonucleotide resulted in the selective ablation of messenger RNA for the double-stranded RNA (dsRNA)-dependent protein kinase PKR. Similarly, purified, recombinant human 2-5A-dependent ribonuclease was induced to selectively cleave PKR messenger RNA. Cells depleted of PKR activity were unresponsive to activation of nuclear factor-κB (NF-κB) by the dsRNA poly(I):poly(C), which provides direct evidence that PKR is a transducer for the dsRNA signaling of NF-κB.
AB - Activation of 2-5A-dependent ribonuclease by 5'-phosphorylated, 2',5'-linked oligoadenylates, known as 2-5A, is one pathway of interferon action. Unaided uptake into HeLa cells of 2-5A linked to an antisense oligonucleotide resulted in the selective ablation of messenger RNA for the double-stranded RNA (dsRNA)-dependent protein kinase PKR. Similarly, purified, recombinant human 2-5A-dependent ribonuclease was induced to selectively cleave PKR messenger RNA. Cells depleted of PKR activity were unresponsive to activation of nuclear factor-κB (NF-κB) by the dsRNA poly(I):poly(C), which provides direct evidence that PKR is a transducer for the dsRNA signaling of NF-κB.
UR - http://www.scopus.com/inward/record.url?scp=0028169329&partnerID=8YFLogxK
U2 - 10.1126/science.7914032
DO - 10.1126/science.7914032
M3 - Article
C2 - 7914032
AN - SCOPUS:0028169329
SN - 0036-8075
VL - 265
SP - 789
EP - 792
JO - Science
JF - Science
IS - 5173
ER -