Blechnum Orientale Linn - a fern with potential as antioxidant, anticancer and antibacterial agent

How Yee Lai, Yau Yan Lim, Kah Kim

    Research output: Contribution to journalArticleResearchpeer-review

    56 Citations (Scopus)

    Abstract

    Background: Blechnum orientale Linn. (Blechnaceae) is used ethnomedicinally for the treatment of various skin diseases, stomach pain, urinary bladder complaints and sterilization of women. The aim of the study was to evaluate antioxidant, anticancer and antibacterial activity of five solvent fractions obtained from the methanol extract of the leaves of Blechnum orientale Linn. Methods: Five solvent fractions were obtained from the methanol extract of B. orientale through successive partitioning with petroleum ether, chloroform, ethyl acetate, butanol and water. Total phenolic content was assessed using Folin-Ciocalteu s method. The antioxidant activity was determined by measuring the scavenging activity of DPPH radicals. Cytotoxic activity was tested against four cancer cell lines and a non-malignant cell using MTT assay. Antibacterial activity was assessed using the disc diffusion and broth microdilution assays. Standard phytochemical screening tests for saponins, tannins, terpenoids, flavonoids and alkaloids were also conducted.
    Original languageEnglish
    Pages (from-to)1 - 8
    Number of pages8
    JournalBMC Complementary and Alternative Medicine
    Volume10
    Issue number15
    DOIs
    Publication statusPublished - 2010

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