DNA methylation analysis is emerging as a new technique with potential capabilities for early cancer detection. However, current state-of-the-art techniques are not easily translatable into routine clinical methods. Herein we describe a bead-based flow cytometry assay which combines DNA hybridization to microparticles with 5MeC-specific proteins/antibodies. These assays can be used to study the binding properties of current and emerging 5MeC-binding proteins and may also have potential in the measurement of 5MeC density in clinical samples for cancer detection.
Corrie, S. R., Sova, P., Feng, Q., Blair, T., Kiviat, N. B., & Trau, M. (2011). Bisulfite free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay. Analyst, 136(4), 688 - 691. https://doi.org/10.1039/C0AN00790K