Bisulfite free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay

Simon R. Corrie, Pavel Sova, Qinghua Feng, Tiffany Blair, Nancy B. Kiviat, Matt Trau

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

DNA methylation analysis is emerging as a new technique with potential capabilities for early cancer detection. However, current state-of-the-art techniques are not easily translatable into routine clinical methods. Herein we describe a bead-based flow cytometry assay which combines DNA hybridization to microparticles with 5MeC-specific proteins/antibodies. These assays can be used to study the binding properties of current and emerging 5MeC-binding proteins and may also have potential in the measurement of 5MeC density in clinical samples for cancer detection.
Original languageEnglish
Pages (from-to)688 - 691
Number of pages4
JournalAnalyst
Volume136
Issue number4
DOIs
Publication statusPublished - 21 Feb 2011
Externally publishedYes

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