Biosensors for detecting stress in developing embryos

Malcolm S. Purdey, Avishkar Saini, Hanna J. McLennan, Benjamin J. Pullen, Erik P. Schartner, Melanie L. Sutton-Mcdowall, Jeremy G. Thompson, Tanya M. Monro, Stephen J. Nicholls, Andrew D. Abell

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearch

Abstract

Reactive Oxygen Species (ROS) cause DNA damage and defective function in sperm and also affects the developmental competence of embryos. It is therefore critical to monitor ROS in sperm, oocytes and developing embryos. In particular, hydrogen peroxide (H2O2) is a ROS important to normal cell function and signalling as well as its role in oxidative stress. Here we report the development of a fluorescent sensor for H2O2 using carboxyperoxyfluor-1 (CPF1) in solution and attached to a glass slide or multi-mode optical fibre. CPF1 increases in fluorescence upon reaction with H2O2 to non-invasively detect H2O2 near developing embryos. These probes are constructed by immobilising CPF1 to the optical fibre tip a polyacrylamide layer. Also reported is a new dual optical fibre sensor for detecting both H2O2 and pH that is functional at biologically concentrations of H2O2 and can sense pH to 0.1 units. This research shows promise for the use of optical fibre sensors for monitoring the health of developing embryos. Furthermore, these sensors are applicable for use beyond embryos such as detecting stress in endothelial cells involved in cardiovascular dysfunction.

Original languageEnglish
Title of host publicationSPIE BioPhotonics Australasia
EditorsEwa M. Goldys, Mark R. Hutchinson
PublisherSPIE
Number of pages6
ISBN (Electronic)9781510604346
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes
EventSPIE BioPhotonics Australasia 2016 - Adelaide Convention Centre, North Terrace, Adelaide, Australia
Duration: 17 Oct 201619 Oct 2016

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
PublisherSPIE
Volume10013
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceSPIE BioPhotonics Australasia 2016
CountryAustralia
CityNorth Terrace, Adelaide
Period17/10/1619/10/16

Cite this

Purdey, M. S., Saini, A., McLennan, H. J., Pullen, B. J., Schartner, E. P., Sutton-Mcdowall, M. L., Thompson, J. G., Monro, T. M., Nicholls, S. J., & Abell, A. D. (2016). Biosensors for detecting stress in developing embryos. In E. M. Goldys, & M. R. Hutchinson (Eds.), SPIE BioPhotonics Australasia [100130B] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10013). SPIE. https://doi.org/10.1117/12.2244645