Biomedical application of Raman and FI-IR spectroscopy: label-free imaging of liver insult

K. Kochan, Malgorzata Baranska, E. Maslak

Research output: Chapter in Book/Report/Conference proceedingEncyclopaedia / Dictionary EntryOtherpeer-review

Abstract

Imaging via vibrational spectroscopy techniques (Raman, Fourier transform infrared spectroscopy, FT‐IR) combined with chemometric analysis is becoming increasingly popular in the field of biomedical research. This results from numerous advantages of FT‐IR and Raman spectroscopies for the study of biological samples and the potential of their application in the context of pathogenesis studies as well as diagnostic use. Each technique, owing to its specificity, has slightly different attributes, which affect the scope of its application (diagnostics vs pathogenesis, micro‐ vs macroscale, tissue vs cellular and subcellular level, etc.) as well as its limitations. Increased recognition of the usefulness of FT‐IR and Raman‐based chemical imaging is visible through the multitude of their applications for various biological samples and a variety of diseases. In this work, we present a review of the adaptation of both techniques for liver‐based research, associated with its most common disorders. We introduce the basics of both techniques with particular emphasis on their advantages and limitations in terms of applications in the field of biomedical research. As the number of successful adaptation of vibrational spectroscopy‐based techniques for biological samples is already tremendous, we focus only on the liver‐related research in the context of lifestyle diseases. The importance and prevalence of lifestyle diseases due to the development of civilization are continually growing. A close connection between liver and lifestyle diseases results from the multitude of liver functions, particularly in terms of metabolism. Lifestyle diseases are linked inseparably with impaired liver function through nonalcoholic fatty liver disease (NAFLD), which can subsequently develop into a multitude of other dysfunctions, including fibrosis, cirrhosis, and cancer.
Original languageEnglish
Title of host publicationEncyclopedia of Analytical Chemistry
EditorsRobert A. Meyers
PublisherJohn Wiley & Sons
Number of pages24
ISBN (Electronic)9780470027318
ISBN (Print)9780471976707
DOIs
Publication statusPublished - 2017
Externally publishedYes

Keywords

  • liver
  • NAFLD
  • lifestyle disease
  • T2DM
  • atherosclerosis
  • cancer
  • fibrosis
  • confocal Raman mapping
  • FT-IR imaging
  • vibrational spectroscopy

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