Biomaterials for spinal cord regeneration: Outgrowth of presumptive neuronal precursors on electrospun poly(ε)-caprolactone scaffolds microlayered with alternating polyelectrolytes

George A. Thouas, Karla G. Contreras, Claude C. Bernard, Kelly Tsang, Kun Zhou, David R. Nisbet, John S. Forsythe, Guizhi Sun

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9 Citations (Scopus)


The aim of this study was to assess the feasibility of electrospun poly(ε)-caprolactone (PCL) scaffolds treated with alternating poly-electrolytes as a controllable three-dimensional adhesive substrate for neuronal progenitors. Unmodified PCL surfaces were generally not supportive of mouse embryonic stem cell (mESC) colony adhesion. However, scaffolds surfaced using layer-by-layer (LbL) deposition of heparin/poly-L-lysine encouraged better local adhesion of mESC colonies, and networking of monolayers containing nestin-positive presumptive neurons, similar to lamin in coated controls, as observed by immuno-fluorescence microscopy. Confocal microscopy further revealed depth-wise penetration of mESC nestin-positive cell populations, and orientation along gross topographical features in the LbL scaffolds. LbL deposition therefore appears to provide a satisfactory adhesive substrate for contact and mechanical guidance of neuroanal outgrowth in three-dimensions.

Original languageEnglish
Title of host publicationProceedings of the 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'08
PublisherIEEE, Institute of Electrical and Electronics Engineers
Number of pages4
ISBN (Print) 978-1-4244-1814-5
Publication statusPublished - 2008
EventInternational Conference of the IEEE Engineering in Medicine and Biology Society 2008 - Vancouver Convention and Exhibition Centre, Vancouver, Canada
Duration: 20 Aug 200825 Aug 2008
Conference number: 30th (Proceedings)


ConferenceInternational Conference of the IEEE Engineering in Medicine and Biology Society 2008
Abbreviated titleEMBS 2008
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