Biomaterials for spinal cord regeneration: Outgrowth of presumptive neuronal precursors on electrospun poly(ε)-caprolactone scaffolds microlayered with alternating polyelectrolytes

George A. Thouas, Karla G. Contreras, Claude C. Bernard, Kelly Tsang, Kun Zhou, David R. Nisbet, John S. Forsythe, Guizhi Sun

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9 Citations (Scopus)

Abstract

The aim of this study was to assess the feasibility of electrospun poly(ε)-caprolactone (PCL) scaffolds treated with alternating poly-electrolytes as a controllable three-dimensional adhesive substrate for neuronal progenitors. Unmodified PCL surfaces were generally not supportive of mouse embryonic stem cell (mESC) colony adhesion. However, scaffolds surfaced using layer-by-layer (LbL) deposition of heparin/poly-L-lysine encouraged better local adhesion of mESC colonies, and networking of monolayers containing nestin-positive presumptive neurons, similar to lamin in coated controls, as observed by immuno-fluorescence microscopy. Confocal microscopy further revealed depth-wise penetration of mESC nestin-positive cell populations, and orientation along gross topographical features in the LbL scaffolds. LbL deposition therefore appears to provide a satisfactory adhesive substrate for contact and mechanical guidance of neuroanal outgrowth in three-dimensions.

Original languageEnglish
Title of host publicationProceedings of the 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'08
PublisherIEEE, Institute of Electrical and Electronics Engineers
Pages1825-1828
Number of pages4
ISBN (Print) 978-1-4244-1814-5
DOIs
Publication statusPublished - 2008
EventInternational Conference of the IEEE Engineering in Medicine and Biology Society 2008 - Vancouver Convention and Exhibition Centre, Vancouver, Canada
Duration: 20 Aug 200825 Aug 2008
Conference number: 30th
https://ieeexplore.ieee.org/xpl/conhome/4636107/proceeding (Proceedings)

Conference

ConferenceInternational Conference of the IEEE Engineering in Medicine and Biology Society 2008
Abbreviated titleEMBS 2008
CountryCanada
CityVancouver
Period20/08/0825/08/08
Internet address

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