Biocompatibility of semiconducting AlGaN/GaN material with living cells

Anna Podolska, Stephanie Tham, Robert D Hart, Ruth Seeber, Martin Kocan, Martina Kocan, Umesh K Mishra, Kevin Pfleger, Giacinta Parish, Brett Douglas Nener

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

With the aim of developing a highly sensitive, mass producible biosensor, we have investigated the growth of human embryonic kidney (HEK) 293 cells on the surface of semiconductor grade AlGaN/GaN heterostructures. Our results demonstrate that, even without specialised surface treatment, a substantial amount of attachment and proliferation of cells is observed. Growth and mortality rates on the AlGaN surface were comparable to standard control culture plates. Quantitative studies of mortality measured by flow cytometry correlate well with qualitative monitoring of biocompatibility. The percentage of dead cells increases marginally with increasing Al concentration. Cell attachment was investigated qualitatively using focused ion beam/scanning electron microscopy (FIB/SEM) and transmission electron microscopy (TEM). Imaging showed strong attachment at the cell/semiconductor interface at the nanometre level. These measurements are the first study of live cell/semiconductor interactions using complementary methods for proliferation, mortality, and attachment, and confirm that the combination of live cells as the biosensing element and AlGaN/GaN heterostructures as the transducer has significant potential for biosensor applications.
Original languageEnglish
Pages (from-to)401 - 406
Number of pages6
JournalSensors and Actuators B: Chemical
Volume169
Issue number5
DOIs
Publication statusPublished - 2012
Externally publishedYes

Cite this

Podolska, A., Tham, S., Hart, R. D., Seeber, R., Kocan, M., Kocan, M., ... Nener, B. D. (2012). Biocompatibility of semiconducting AlGaN/GaN material with living cells. Sensors and Actuators B: Chemical, 169(5), 401 - 406. https://doi.org/10.1016/j.snb.2012.04.015
Podolska, Anna ; Tham, Stephanie ; Hart, Robert D ; Seeber, Ruth ; Kocan, Martin ; Kocan, Martina ; Mishra, Umesh K ; Pfleger, Kevin ; Parish, Giacinta ; Nener, Brett Douglas. / Biocompatibility of semiconducting AlGaN/GaN material with living cells. In: Sensors and Actuators B: Chemical. 2012 ; Vol. 169, No. 5. pp. 401 - 406.
@article{9220923d1b514c4db37b250e935406ec,
title = "Biocompatibility of semiconducting AlGaN/GaN material with living cells",
abstract = "With the aim of developing a highly sensitive, mass producible biosensor, we have investigated the growth of human embryonic kidney (HEK) 293 cells on the surface of semiconductor grade AlGaN/GaN heterostructures. Our results demonstrate that, even without specialised surface treatment, a substantial amount of attachment and proliferation of cells is observed. Growth and mortality rates on the AlGaN surface were comparable to standard control culture plates. Quantitative studies of mortality measured by flow cytometry correlate well with qualitative monitoring of biocompatibility. The percentage of dead cells increases marginally with increasing Al concentration. Cell attachment was investigated qualitatively using focused ion beam/scanning electron microscopy (FIB/SEM) and transmission electron microscopy (TEM). Imaging showed strong attachment at the cell/semiconductor interface at the nanometre level. These measurements are the first study of live cell/semiconductor interactions using complementary methods for proliferation, mortality, and attachment, and confirm that the combination of live cells as the biosensing element and AlGaN/GaN heterostructures as the transducer has significant potential for biosensor applications.",
author = "Anna Podolska and Stephanie Tham and Hart, {Robert D} and Ruth Seeber and Martin Kocan and Martina Kocan and Mishra, {Umesh K} and Kevin Pfleger and Giacinta Parish and Nener, {Brett Douglas}",
year = "2012",
doi = "10.1016/j.snb.2012.04.015",
language = "English",
volume = "169",
pages = "401 -- 406",
journal = "Sensors and Actuators B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "5",

}

Podolska, A, Tham, S, Hart, RD, Seeber, R, Kocan, M, Kocan, M, Mishra, UK, Pfleger, K, Parish, G & Nener, BD 2012, 'Biocompatibility of semiconducting AlGaN/GaN material with living cells', Sensors and Actuators B: Chemical, vol. 169, no. 5, pp. 401 - 406. https://doi.org/10.1016/j.snb.2012.04.015

Biocompatibility of semiconducting AlGaN/GaN material with living cells. / Podolska, Anna; Tham, Stephanie; Hart, Robert D; Seeber, Ruth; Kocan, Martin; Kocan, Martina; Mishra, Umesh K; Pfleger, Kevin; Parish, Giacinta; Nener, Brett Douglas.

In: Sensors and Actuators B: Chemical, Vol. 169, No. 5, 2012, p. 401 - 406.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Biocompatibility of semiconducting AlGaN/GaN material with living cells

AU - Podolska, Anna

AU - Tham, Stephanie

AU - Hart, Robert D

AU - Seeber, Ruth

AU - Kocan, Martin

AU - Kocan, Martina

AU - Mishra, Umesh K

AU - Pfleger, Kevin

AU - Parish, Giacinta

AU - Nener, Brett Douglas

PY - 2012

Y1 - 2012

N2 - With the aim of developing a highly sensitive, mass producible biosensor, we have investigated the growth of human embryonic kidney (HEK) 293 cells on the surface of semiconductor grade AlGaN/GaN heterostructures. Our results demonstrate that, even without specialised surface treatment, a substantial amount of attachment and proliferation of cells is observed. Growth and mortality rates on the AlGaN surface were comparable to standard control culture plates. Quantitative studies of mortality measured by flow cytometry correlate well with qualitative monitoring of biocompatibility. The percentage of dead cells increases marginally with increasing Al concentration. Cell attachment was investigated qualitatively using focused ion beam/scanning electron microscopy (FIB/SEM) and transmission electron microscopy (TEM). Imaging showed strong attachment at the cell/semiconductor interface at the nanometre level. These measurements are the first study of live cell/semiconductor interactions using complementary methods for proliferation, mortality, and attachment, and confirm that the combination of live cells as the biosensing element and AlGaN/GaN heterostructures as the transducer has significant potential for biosensor applications.

AB - With the aim of developing a highly sensitive, mass producible biosensor, we have investigated the growth of human embryonic kidney (HEK) 293 cells on the surface of semiconductor grade AlGaN/GaN heterostructures. Our results demonstrate that, even without specialised surface treatment, a substantial amount of attachment and proliferation of cells is observed. Growth and mortality rates on the AlGaN surface were comparable to standard control culture plates. Quantitative studies of mortality measured by flow cytometry correlate well with qualitative monitoring of biocompatibility. The percentage of dead cells increases marginally with increasing Al concentration. Cell attachment was investigated qualitatively using focused ion beam/scanning electron microscopy (FIB/SEM) and transmission electron microscopy (TEM). Imaging showed strong attachment at the cell/semiconductor interface at the nanometre level. These measurements are the first study of live cell/semiconductor interactions using complementary methods for proliferation, mortality, and attachment, and confirm that the combination of live cells as the biosensing element and AlGaN/GaN heterostructures as the transducer has significant potential for biosensor applications.

UR - http://www.sciencedirect.com/science/article/pii/S0925400512003644#

U2 - 10.1016/j.snb.2012.04.015

DO - 10.1016/j.snb.2012.04.015

M3 - Article

VL - 169

SP - 401

EP - 406

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

SN - 0925-4005

IS - 5

ER -