Biochemical and toxinological characterization of Naja sumatrana (Equatorial spitting cobra) venom

M. K.K. Yap, N. H. Tan, S. Y. Fung

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Abstract

The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkaline phosphomonoesterase and L-amino acid oxidase activities, moderately high acetylcholinesterase and hyaluronidase activities and high phospholipase A 2. Fractionation of N. sumatrana venom by Resource ® S cation exchange chromatography (GE Healthcare, USA) yielded nine major protein peaks, with all except the acidic protein peak being lethal to mice.
Original languageEnglish
Pages (from-to)451-459
Number of pages9
JournalJournal of Venomous Animals and Toxins Including Tropical Diseases
Volume17
Issue number4
Publication statusPublished - 2011
Externally publishedYes

Keywords

  • Cardiotoxin
  • Cobra venom
  • Enzyme activity
  • Naja

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