TY - JOUR
T1 - Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana korth
AU - Chew, Yik Ling
AU - Lim, Yau Yan
AU - Stanslas, Johnson
AU - Ee, Gwendoline Cheng Lian
AU - Goh, Joo Kheng
PY - 2014
Y1 - 2014
N2 - Background: Flowers of Bauhinia kockiana were investigated for their anticancer properties. Methods: Gallic acid (1), and methyl gallate (2), were isolated via bioassay-directed isolation, and they exhibited anticancer properties towards several cancer cell lines, examined using MTT cell viability assay. Pyrogallol (3) was examined against the same cancer cell lines to deduce the bioactive functional group of the phenolic compounds. Results: The results showed that the phenolic compounds could exhibit moderate to weak cytotoxicity towards certain cell lines (GI50 30 - 86 mu M), but were inactive towards DU145 prostate cancer cell (GI50 > 100 mu M). Conclusion: It was observed that pyrogallol moiety was one of the essential functional structures of the phenolic compounds in exhibiting anticancer activity. Also, the carboxyl group of compound (1) was also important in anticancer activity. Examination of the PC-3 cells treated with compound (1) using fluorescence microscopy showed that PC-3 cells were killed by apoptosis.
AB - Background: Flowers of Bauhinia kockiana were investigated for their anticancer properties. Methods: Gallic acid (1), and methyl gallate (2), were isolated via bioassay-directed isolation, and they exhibited anticancer properties towards several cancer cell lines, examined using MTT cell viability assay. Pyrogallol (3) was examined against the same cancer cell lines to deduce the bioactive functional group of the phenolic compounds. Results: The results showed that the phenolic compounds could exhibit moderate to weak cytotoxicity towards certain cell lines (GI50 30 - 86 mu M), but were inactive towards DU145 prostate cancer cell (GI50 > 100 mu M). Conclusion: It was observed that pyrogallol moiety was one of the essential functional structures of the phenolic compounds in exhibiting anticancer activity. Also, the carboxyl group of compound (1) was also important in anticancer activity. Examination of the PC-3 cells treated with compound (1) using fluorescence microscopy showed that PC-3 cells were killed by apoptosis.
UR - https://www.scopus.com/pages/publications/84964315915
U2 - 10.4314/ajtcam.v11i3.40
DO - 10.4314/ajtcam.v11i3.40
M3 - Article
SN - 0189-6016
VL - 11
SP - 291
EP - 299
JO - African Journal of Traditional, Complementary and Alternative Medicines
JF - African Journal of Traditional, Complementary and Alternative Medicines
IS - 3
ER -
