Bioaccumulation on HVAC filters in university buildings in Singapore

Irvan Luhung, Yan Wu, Victor W.C. Chang, William W. Nazaroff

Research output: Chapter in Book/Report/Conference proceedingConference PaperOther

Abstract

Particle filters are important components of HVAC systems. Biomass accumulation on HVAC filters can potentially have adverse effects on building occupants. The research reported in this paper aims to quantitatively describe the content of biomass on HVAC filters from various indoor office environments in Singapore in relation to key governing processes. In this initial phase, filter samples were collected and analysed using DNA-based methods. The collected DNA was directly extracted from the filters and the final concentration was measured with Qubit and qPCR methods. The DNA concentrations were then related to the cumulative indoor occupancy level. The results show good correlations between bacterial DNA and cumulative occupancy. The proportion of total DNA that belongs to fungal and bacterial species decreases as occupancy level increases, probably because of biomass from other sources such as human skin cells.

Original languageEnglish
Title of host publicationProceedings of the Healthy Buildings 2015: America Conference - Innovation in a Time of Energy Uncertainty and Climate Adaptation
Pages90-93
Number of pages4
Publication statusPublished - 2015
Externally publishedYes
EventHealthy Buildings 2015: America Conference - Innovation in a Time of Energy Uncertainty and Climate Adaptation - Boulder, United States of America
Duration: 19 Jul 201522 Jul 2015

Conference

ConferenceHealthy Buildings 2015
Abbreviated titleHB 2015
CountryUnited States of America
CityBoulder
Period19/07/1522/07/15

Keywords

  • DNA-based analysis
  • HVAC filter
  • Occupancy level
  • University buildings

Cite this

Luhung, I., Wu, Y., Chang, V. W. C., & Nazaroff, W. W. (2015). Bioaccumulation on HVAC filters in university buildings in Singapore. In Proceedings of the Healthy Buildings 2015: America Conference - Innovation in a Time of Energy Uncertainty and Climate Adaptation (pp. 90-93)
Luhung, Irvan ; Wu, Yan ; Chang, Victor W.C. ; Nazaroff, William W. / Bioaccumulation on HVAC filters in university buildings in Singapore. Proceedings of the Healthy Buildings 2015: America Conference - Innovation in a Time of Energy Uncertainty and Climate Adaptation. 2015. pp. 90-93
@inproceedings{4e2d48e1d64b4a6b99d90960fae1d33c,
title = "Bioaccumulation on HVAC filters in university buildings in Singapore",
abstract = "Particle filters are important components of HVAC systems. Biomass accumulation on HVAC filters can potentially have adverse effects on building occupants. The research reported in this paper aims to quantitatively describe the content of biomass on HVAC filters from various indoor office environments in Singapore in relation to key governing processes. In this initial phase, filter samples were collected and analysed using DNA-based methods. The collected DNA was directly extracted from the filters and the final concentration was measured with Qubit and qPCR methods. The DNA concentrations were then related to the cumulative indoor occupancy level. The results show good correlations between bacterial DNA and cumulative occupancy. The proportion of total DNA that belongs to fungal and bacterial species decreases as occupancy level increases, probably because of biomass from other sources such as human skin cells.",
keywords = "DNA-based analysis, HVAC filter, Occupancy level, University buildings",
author = "Irvan Luhung and Yan Wu and Chang, {Victor W.C.} and Nazaroff, {William W.}",
year = "2015",
language = "English",
pages = "90--93",
booktitle = "Proceedings of the Healthy Buildings 2015: America Conference - Innovation in a Time of Energy Uncertainty and Climate Adaptation",

}

Luhung, I, Wu, Y, Chang, VWC & Nazaroff, WW 2015, Bioaccumulation on HVAC filters in university buildings in Singapore. in Proceedings of the Healthy Buildings 2015: America Conference - Innovation in a Time of Energy Uncertainty and Climate Adaptation. pp. 90-93, Healthy Buildings 2015, Boulder, United States of America, 19/07/15.

Bioaccumulation on HVAC filters in university buildings in Singapore. / Luhung, Irvan; Wu, Yan; Chang, Victor W.C.; Nazaroff, William W.

Proceedings of the Healthy Buildings 2015: America Conference - Innovation in a Time of Energy Uncertainty and Climate Adaptation. 2015. p. 90-93.

Research output: Chapter in Book/Report/Conference proceedingConference PaperOther

TY - GEN

T1 - Bioaccumulation on HVAC filters in university buildings in Singapore

AU - Luhung, Irvan

AU - Wu, Yan

AU - Chang, Victor W.C.

AU - Nazaroff, William W.

PY - 2015

Y1 - 2015

N2 - Particle filters are important components of HVAC systems. Biomass accumulation on HVAC filters can potentially have adverse effects on building occupants. The research reported in this paper aims to quantitatively describe the content of biomass on HVAC filters from various indoor office environments in Singapore in relation to key governing processes. In this initial phase, filter samples were collected and analysed using DNA-based methods. The collected DNA was directly extracted from the filters and the final concentration was measured with Qubit and qPCR methods. The DNA concentrations were then related to the cumulative indoor occupancy level. The results show good correlations between bacterial DNA and cumulative occupancy. The proportion of total DNA that belongs to fungal and bacterial species decreases as occupancy level increases, probably because of biomass from other sources such as human skin cells.

AB - Particle filters are important components of HVAC systems. Biomass accumulation on HVAC filters can potentially have adverse effects on building occupants. The research reported in this paper aims to quantitatively describe the content of biomass on HVAC filters from various indoor office environments in Singapore in relation to key governing processes. In this initial phase, filter samples were collected and analysed using DNA-based methods. The collected DNA was directly extracted from the filters and the final concentration was measured with Qubit and qPCR methods. The DNA concentrations were then related to the cumulative indoor occupancy level. The results show good correlations between bacterial DNA and cumulative occupancy. The proportion of total DNA that belongs to fungal and bacterial species decreases as occupancy level increases, probably because of biomass from other sources such as human skin cells.

KW - DNA-based analysis

KW - HVAC filter

KW - Occupancy level

KW - University buildings

UR - http://www.scopus.com/inward/record.url?scp=85052395782&partnerID=8YFLogxK

M3 - Conference Paper

SP - 90

EP - 93

BT - Proceedings of the Healthy Buildings 2015: America Conference - Innovation in a Time of Energy Uncertainty and Climate Adaptation

ER -

Luhung I, Wu Y, Chang VWC, Nazaroff WW. Bioaccumulation on HVAC filters in university buildings in Singapore. In Proceedings of the Healthy Buildings 2015: America Conference - Innovation in a Time of Energy Uncertainty and Climate Adaptation. 2015. p. 90-93