Bio-recognition molecules onto nano-crystals of carbonate apatite for cell-targeted gene delivery and expression

E. H. Chowdhury, K. Megumi, I. Harada, A. K. Kundu, T. Hoshiba, A. Maruyama, T. Akaike

Research output: Chapter in Book/Report/Conference proceedingConference PaperOtherpeer-review

Abstract

The development of nano-particles of DNA/carbonate apatite having virus-like properties that enables the effective delivery of a foreign DNA to a specific organ was investigated. The DNA or carbonate apatite particles were incubated with a specific cell-recognizable protein for 30 min at 37°C and then further incubated with bovine serum albumin (BSA). Particles upon coming in contact with serum proteins or BSA showed microscopically visible brownian motion. By using galactose-specific lectin, it was shown that asialofetuin having triantennary galactose terminated sugar chains, could still present galactose residues efficiently while being absorbed onto the crystals along with BSA.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages1805
Number of pages1
Publication statusPublished - 2004
Externally publishedYes
EventWorld Biomaterials Congress (WBC) 2004 - Sydney, Australia
Duration: 17 May 200421 May 2004
Conference number: 7th

Conference

ConferenceWorld Biomaterials Congress (WBC) 2004
CountryAustralia
CitySydney
Period17/05/0421/05/04

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