Abstract
Surface-Enhanced Raman Scattering (SERS) is emerging as a promising strategy for the quantification of immunoglobulin G (IgG) due to its inherent high sensitivity and specificity; however, it remains challenging to integrate SERS detection with a microfluidic system in a simple, efficient and low-cost manner. Here, we report on a novel bifunctional plasmonic-magnetic particle-based immunoassay, in which plasmonic nanoparticles act as soluble SERS immunosubstrates, whereas magnetic particles are for promoting micromixing in a microfluidic chip. With this novel SERS immunosubstrate in conjunction with the unique microfluidic system, we could substantially reduce the assay time from 4 hours to 80 minutes as well as enhance the detection specificity by about 70% in comparison to a non-microfluidic immunoassay. Compared to previous microfluidic SERS systems, our strategy offers a simple microfluidic chip design with only one well for mixing, washing and detection.
Original language | English |
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Pages (from-to) | 7822-7829 |
Number of pages | 8 |
Journal | Nanoscale |
Volume | 9 |
Issue number | 23 |
DOIs | |
Publication status | Published - 21 Jun 2017 |
Equipment
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Melbourne Centre for Nanofabrication
Sean Langelier (Manager)
Office of the Vice-Provost (Research and Research Infrastructure)Facility/equipment: Facility