Bifunctional plasmonic-magnetic particles for an enhanced microfluidic SERS immunoassay

Lim Wei Yap, Huaying Chen, Yuan Gao, Karolina Petkovic, Yan Liang, Kae Jye Si, Huanting Wang, Zhiyong Tang, Yonggang Zhu, Wenlong Cheng

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

Surface-Enhanced Raman Scattering (SERS) is emerging as a promising strategy for the quantification of immunoglobulin G (IgG) due to its inherent high sensitivity and specificity; however, it remains challenging to integrate SERS detection with a microfluidic system in a simple, efficient and low-cost manner. Here, we report on a novel bifunctional plasmonic-magnetic particle-based immunoassay, in which plasmonic nanoparticles act as soluble SERS immunosubstrates, whereas magnetic particles are for promoting micromixing in a microfluidic chip. With this novel SERS immunosubstrate in conjunction with the unique microfluidic system, we could substantially reduce the assay time from 4 hours to 80 minutes as well as enhance the detection specificity by about 70% in comparison to a non-microfluidic immunoassay. Compared to previous microfluidic SERS systems, our strategy offers a simple microfluidic chip design with only one well for mixing, washing and detection.

Original languageEnglish
Pages (from-to)7822-7829
Number of pages8
JournalNanoscale
Volume9
Issue number23
DOIs
Publication statusPublished - 21 Jun 2017

Cite this

Yap, Lim Wei ; Chen, Huaying ; Gao, Yuan ; Petkovic, Karolina ; Liang, Yan ; Si, Kae Jye ; Wang, Huanting ; Tang, Zhiyong ; Zhu, Yonggang ; Cheng, Wenlong. / Bifunctional plasmonic-magnetic particles for an enhanced microfluidic SERS immunoassay. In: Nanoscale. 2017 ; Vol. 9, No. 23. pp. 7822-7829.
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abstract = "Surface-Enhanced Raman Scattering (SERS) is emerging as a promising strategy for the quantification of immunoglobulin G (IgG) due to its inherent high sensitivity and specificity; however, it remains challenging to integrate SERS detection with a microfluidic system in a simple, efficient and low-cost manner. Here, we report on a novel bifunctional plasmonic-magnetic particle-based immunoassay, in which plasmonic nanoparticles act as soluble SERS immunosubstrates, whereas magnetic particles are for promoting micromixing in a microfluidic chip. With this novel SERS immunosubstrate in conjunction with the unique microfluidic system, we could substantially reduce the assay time from 4 hours to 80 minutes as well as enhance the detection specificity by about 70{\%} in comparison to a non-microfluidic immunoassay. Compared to previous microfluidic SERS systems, our strategy offers a simple microfluidic chip design with only one well for mixing, washing and detection.",
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Yap, LW, Chen, H, Gao, Y, Petkovic, K, Liang, Y, Si, KJ, Wang, H, Tang, Z, Zhu, Y & Cheng, W 2017, 'Bifunctional plasmonic-magnetic particles for an enhanced microfluidic SERS immunoassay', Nanoscale, vol. 9, no. 23, pp. 7822-7829. https://doi.org/10.1039/c7nr01511a

Bifunctional plasmonic-magnetic particles for an enhanced microfluidic SERS immunoassay. / Yap, Lim Wei; Chen, Huaying; Gao, Yuan; Petkovic, Karolina; Liang, Yan; Si, Kae Jye; Wang, Huanting; Tang, Zhiyong; Zhu, Yonggang; Cheng, Wenlong.

In: Nanoscale, Vol. 9, No. 23, 21.06.2017, p. 7822-7829.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Chen, Huaying

AU - Gao, Yuan

AU - Petkovic, Karolina

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AU - Si, Kae Jye

AU - Wang, Huanting

AU - Tang, Zhiyong

AU - Zhu, Yonggang

AU - Cheng, Wenlong

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