Abstract
Surface-Enhanced Raman Scattering (SERS) is emerging as a promising strategy for the quantification of immunoglobulin G (IgG) due to its inherent high sensitivity and specificity; however, it remains challenging to integrate SERS detection with a microfluidic system in a simple, efficient and low-cost manner. Here, we report on a novel bifunctional plasmonic-magnetic particle-based immunoassay, in which plasmonic nanoparticles act as soluble SERS immunosubstrates, whereas magnetic particles are for promoting micromixing in a microfluidic chip. With this novel SERS immunosubstrate in conjunction with the unique microfluidic system, we could substantially reduce the assay time from 4 hours to 80 minutes as well as enhance the detection specificity by about 70% in comparison to a non-microfluidic immunoassay. Compared to previous microfluidic SERS systems, our strategy offers a simple microfluidic chip design with only one well for mixing, washing and detection.
| Original language | English |
|---|---|
| Pages (from-to) | 7822-7829 |
| Number of pages | 8 |
| Journal | Nanoscale |
| Volume | 9 |
| Issue number | 23 |
| DOIs | |
| Publication status | Published - 21 Jun 2017 |
Cite this
}
Bifunctional plasmonic-magnetic particles for an enhanced microfluidic SERS immunoassay. / Yap, Lim Wei; Chen, Huaying; Gao, Yuan; Petkovic, Karolina; Liang, Yan; Si, Kae Jye; Wang, Huanting; Tang, Zhiyong; Zhu, Yonggang; Cheng, Wenlong.
In: Nanoscale, Vol. 9, No. 23, 21.06.2017, p. 7822-7829.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Bifunctional plasmonic-magnetic particles for an enhanced microfluidic SERS immunoassay
AU - Yap, Lim Wei
AU - Chen, Huaying
AU - Gao, Yuan
AU - Petkovic, Karolina
AU - Liang, Yan
AU - Si, Kae Jye
AU - Wang, Huanting
AU - Tang, Zhiyong
AU - Zhu, Yonggang
AU - Cheng, Wenlong
PY - 2017/6/21
Y1 - 2017/6/21
N2 - Surface-Enhanced Raman Scattering (SERS) is emerging as a promising strategy for the quantification of immunoglobulin G (IgG) due to its inherent high sensitivity and specificity; however, it remains challenging to integrate SERS detection with a microfluidic system in a simple, efficient and low-cost manner. Here, we report on a novel bifunctional plasmonic-magnetic particle-based immunoassay, in which plasmonic nanoparticles act as soluble SERS immunosubstrates, whereas magnetic particles are for promoting micromixing in a microfluidic chip. With this novel SERS immunosubstrate in conjunction with the unique microfluidic system, we could substantially reduce the assay time from 4 hours to 80 minutes as well as enhance the detection specificity by about 70% in comparison to a non-microfluidic immunoassay. Compared to previous microfluidic SERS systems, our strategy offers a simple microfluidic chip design with only one well for mixing, washing and detection.
AB - Surface-Enhanced Raman Scattering (SERS) is emerging as a promising strategy for the quantification of immunoglobulin G (IgG) due to its inherent high sensitivity and specificity; however, it remains challenging to integrate SERS detection with a microfluidic system in a simple, efficient and low-cost manner. Here, we report on a novel bifunctional plasmonic-magnetic particle-based immunoassay, in which plasmonic nanoparticles act as soluble SERS immunosubstrates, whereas magnetic particles are for promoting micromixing in a microfluidic chip. With this novel SERS immunosubstrate in conjunction with the unique microfluidic system, we could substantially reduce the assay time from 4 hours to 80 minutes as well as enhance the detection specificity by about 70% in comparison to a non-microfluidic immunoassay. Compared to previous microfluidic SERS systems, our strategy offers a simple microfluidic chip design with only one well for mixing, washing and detection.
UR - http://www.scopus.com/inward/record.url?scp=85021787877&partnerID=8YFLogxK
U2 - 10.1039/c7nr01511a
DO - 10.1039/c7nr01511a
M3 - Article
VL - 9
SP - 7822
EP - 7829
JO - Nanoscale
JF - Nanoscale
SN - 2040-3364
IS - 23
ER -