Biasing amacrine subtypes in the Atoh7 lineage through expression of barhl2

Patricia R Jusuf, Shahad Albadri, Alessio Paolini, Peter D Currie, Francesco Argenton, Shin-ichi Higashijima, William A Harris, Lucia Poggi

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

Within the developing vertebrate retina, particular subtypes of amacrine cells (ACs) tend to arise from progenitors expressing the basic helix-loop-helix (bHLH) transcription factor, Atoh7, which is necessary for the early generation of retinal ganglion cells (RGCs). All ACs require the postmitotic expression of the bHLH pancreas transcription factor Ptf1a; however, Ptf1a alone is not sufficient to give subtype identities. Here we use functional and in vivo time-lapse studies in the zebrafish retina to investigate on the developmental programs leading to ACs specification within the subsequent divisions of Atoh7-positive progenitors. We find evidences that the homeobox transcription factor Barhl2 is an AC subtype identity-biasing factor that turns on within Atoh7-positive descendants. In vivo lineage tracing reveals that particular modes of cell division tend to generate Barhl2-positive precursors from sisters of RGCs. Additionally, Atoh7 indirectly impacts these division modes to regulate the right number of barhl2-expressing cells. We finally find that Atoh7 itself influences the subtypes of Barhl2-dependent ACs. Together, the results from our study uncover lineage-related and molecular logic of subtype specification in the vertebrate retina, by showing that specific AC subtypes arise via a particular mode of cell division and a transcriptional network cascade involving the sequential expression of first atoh7 followed by ptf1a and then barhl2.
Original languageEnglish
Pages (from-to)13929 - 13944
Number of pages16
JournalJournal of Neuroscience
Volume32
Issue number40
DOIs
Publication statusPublished - 2012

Cite this

Jusuf, Patricia R ; Albadri, Shahad ; Paolini, Alessio ; Currie, Peter D ; Argenton, Francesco ; Higashijima, Shin-ichi ; Harris, William A ; Poggi, Lucia. / Biasing amacrine subtypes in the Atoh7 lineage through expression of barhl2. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 40. pp. 13929 - 13944.
@article{1ca390f821bb400f98865dd8029cc9d5,
title = "Biasing amacrine subtypes in the Atoh7 lineage through expression of barhl2",
abstract = "Within the developing vertebrate retina, particular subtypes of amacrine cells (ACs) tend to arise from progenitors expressing the basic helix-loop-helix (bHLH) transcription factor, Atoh7, which is necessary for the early generation of retinal ganglion cells (RGCs). All ACs require the postmitotic expression of the bHLH pancreas transcription factor Ptf1a; however, Ptf1a alone is not sufficient to give subtype identities. Here we use functional and in vivo time-lapse studies in the zebrafish retina to investigate on the developmental programs leading to ACs specification within the subsequent divisions of Atoh7-positive progenitors. We find evidences that the homeobox transcription factor Barhl2 is an AC subtype identity-biasing factor that turns on within Atoh7-positive descendants. In vivo lineage tracing reveals that particular modes of cell division tend to generate Barhl2-positive precursors from sisters of RGCs. Additionally, Atoh7 indirectly impacts these division modes to regulate the right number of barhl2-expressing cells. We finally find that Atoh7 itself influences the subtypes of Barhl2-dependent ACs. Together, the results from our study uncover lineage-related and molecular logic of subtype specification in the vertebrate retina, by showing that specific AC subtypes arise via a particular mode of cell division and a transcriptional network cascade involving the sequential expression of first atoh7 followed by ptf1a and then barhl2.",
author = "Jusuf, {Patricia R} and Shahad Albadri and Alessio Paolini and Currie, {Peter D} and Francesco Argenton and Shin-ichi Higashijima and Harris, {William A} and Lucia Poggi",
year = "2012",
doi = "10.1523/JNEUROSCI.2073-12.2012",
language = "English",
volume = "32",
pages = "13929 -- 13944",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "40",

}

Jusuf, PR, Albadri, S, Paolini, A, Currie, PD, Argenton, F, Higashijima, S, Harris, WA & Poggi, L 2012, 'Biasing amacrine subtypes in the Atoh7 lineage through expression of barhl2', Journal of Neuroscience, vol. 32, no. 40, pp. 13929 - 13944. https://doi.org/10.1523/JNEUROSCI.2073-12.2012

Biasing amacrine subtypes in the Atoh7 lineage through expression of barhl2. / Jusuf, Patricia R; Albadri, Shahad; Paolini, Alessio; Currie, Peter D; Argenton, Francesco; Higashijima, Shin-ichi; Harris, William A; Poggi, Lucia.

In: Journal of Neuroscience, Vol. 32, No. 40, 2012, p. 13929 - 13944.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Biasing amacrine subtypes in the Atoh7 lineage through expression of barhl2

AU - Jusuf, Patricia R

AU - Albadri, Shahad

AU - Paolini, Alessio

AU - Currie, Peter D

AU - Argenton, Francesco

AU - Higashijima, Shin-ichi

AU - Harris, William A

AU - Poggi, Lucia

PY - 2012

Y1 - 2012

N2 - Within the developing vertebrate retina, particular subtypes of amacrine cells (ACs) tend to arise from progenitors expressing the basic helix-loop-helix (bHLH) transcription factor, Atoh7, which is necessary for the early generation of retinal ganglion cells (RGCs). All ACs require the postmitotic expression of the bHLH pancreas transcription factor Ptf1a; however, Ptf1a alone is not sufficient to give subtype identities. Here we use functional and in vivo time-lapse studies in the zebrafish retina to investigate on the developmental programs leading to ACs specification within the subsequent divisions of Atoh7-positive progenitors. We find evidences that the homeobox transcription factor Barhl2 is an AC subtype identity-biasing factor that turns on within Atoh7-positive descendants. In vivo lineage tracing reveals that particular modes of cell division tend to generate Barhl2-positive precursors from sisters of RGCs. Additionally, Atoh7 indirectly impacts these division modes to regulate the right number of barhl2-expressing cells. We finally find that Atoh7 itself influences the subtypes of Barhl2-dependent ACs. Together, the results from our study uncover lineage-related and molecular logic of subtype specification in the vertebrate retina, by showing that specific AC subtypes arise via a particular mode of cell division and a transcriptional network cascade involving the sequential expression of first atoh7 followed by ptf1a and then barhl2.

AB - Within the developing vertebrate retina, particular subtypes of amacrine cells (ACs) tend to arise from progenitors expressing the basic helix-loop-helix (bHLH) transcription factor, Atoh7, which is necessary for the early generation of retinal ganglion cells (RGCs). All ACs require the postmitotic expression of the bHLH pancreas transcription factor Ptf1a; however, Ptf1a alone is not sufficient to give subtype identities. Here we use functional and in vivo time-lapse studies in the zebrafish retina to investigate on the developmental programs leading to ACs specification within the subsequent divisions of Atoh7-positive progenitors. We find evidences that the homeobox transcription factor Barhl2 is an AC subtype identity-biasing factor that turns on within Atoh7-positive descendants. In vivo lineage tracing reveals that particular modes of cell division tend to generate Barhl2-positive precursors from sisters of RGCs. Additionally, Atoh7 indirectly impacts these division modes to regulate the right number of barhl2-expressing cells. We finally find that Atoh7 itself influences the subtypes of Barhl2-dependent ACs. Together, the results from our study uncover lineage-related and molecular logic of subtype specification in the vertebrate retina, by showing that specific AC subtypes arise via a particular mode of cell division and a transcriptional network cascade involving the sequential expression of first atoh7 followed by ptf1a and then barhl2.

UR - http://www.jneurosci.org/content/32/40/13929.full.pdf

U2 - 10.1523/JNEUROSCI.2073-12.2012

DO - 10.1523/JNEUROSCI.2073-12.2012

M3 - Article

VL - 32

SP - 13929

EP - 13944

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 40

ER -