Betacyanins from Hylocereus polyrhizus: pectinase-assisted extraction and application as a natural food colourant in ice cream

Ashwini Gengatharan, Gary Dykes, Wee Sim Choo

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

The effect of solvent, substrate-to-solvent ratio and concentration of pectinase on the extraction of betacyanins from the pulp of red pitahaya (Hylocereus polyrhizus) was evaluated with respect to yield, betacyanin content (BC) and total sugar content. The application of betacyanins from red pitahaya in ice cream was then evaluated by comparison to a commercial colourant, E-162. Without the use of pectinase, the highest yields (9.11 ± 0.35%) of betacyanins were obtained using 95% ethanol at a substrate-to-solvent ratio of 1:1. With the use of pectinase at a concentration of 1.5%, the highest yield (17.11–17.45%) of betacyanins were obtained using water as a solvent at a substrate-to-solvent ratio of 1:1 and 1:2. Pectinase treatment (1.5–2.5%) using water as a solvent yielded betacyanins with the highest BC (126.47–130.83 g kg−1) and lowest total sugar content (57.85–59.74 g kg−1). The BC and total colour changes were similar in ice cream containing betacyanins from red pitahaya and E-162 throughout the 21-days of frozen storage at −18 °C. Betacyanins from red pitahaya or E-162 enhanced the antioxidant properties of ice cream. The sensory evaluation of ice cream containing betacyanins from red pitahaya showed a better colour acceptability than E-162.

Original languageEnglish
Pages (from-to)1401-1410
Number of pages10
JournalJournal of Food Science and Technology
Volume58
Issue number4
DOIs
Publication statusPublished - Apr 2021

Keywords

  • Betalain
  • Colour
  • E162
  • Pigment
  • Red dragon fruit
  • Red pitahaya

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