Beta-cyclodextrin decorated nanostructured SERS substrates facilitate selective detection of endocrine disruptor chemicals

Cheng Fang, Narasimha Murthy Bandaru, Amanda Vera Ellis, Nicolas Hans Voelcker

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41 Citations (Scopus)


We demonstrate the selective detection of endocrine disruptor chemicals (EDCs) from river water using surface enhanced Raman scattering (SERS). By means of nanosphere lithography, the SERS substrate was prepared via the initial deposition of a monolayer of silica nanospheres (with diameter of ~330. nm) on a silicon substrate as the template. Subsequently, a 180. nm thick layer of silver followed by a 20. nm layer of gold was deposited. This surface was modified with mono-6-deoxy-6-((2-mercaptoethyl)amino)-beta-cyclodextrin (Β-CD) in order to produce a selective capture surface suitable for EDC capture and their detection by means of SERS. We show that EDC model compounds, including 3-amino-2-naphthoic acid (NAPH), potassium hydrogen phthalate (PHTH) and the EDC Β-estradiol (ESTR), were captured by the Β-CD decorated surface. This surface facilitated SERS detection with limits of detection of 3.0. μM (NAPH), 10. μM (PHTH) and 300. nM (ESTR), all 10-100 times lower than that without the surface modification with Β-CD. Individual and simultaneous detection of NAPH and PHTH from their mixture was achieved as evidenced using the bianalyte Raman technique.

Original languageEnglish
Pages (from-to)632-639
Number of pages8
JournalBiosensors and Bioelectronics
Issue number1
Publication statusPublished - 5 Apr 2013
Externally publishedYes


  • Beta-cyclodextrin
  • Endocrine disruptor chemicals
  • Nanosphere lithography
  • Surface enhanced Raman scattering

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