Abstract
A major goal of evolutionary biology is to understand how beneficial mutations translate into increased fitness. Here, we study beneficial mutations that arise in experimental populations of yeast evolved in glucose-rich media. We find that fitness increases are caused by enhanced maximum growth rate (R) that come at the cost of reduced yield (K). We show that for some of these mutants, high R coincides with higher rates of ethanol secretion, suggesting that higher growth rates are due to an increased preference to utilize glucose through the fermentation pathway, instead of respiration. We examine the performance of mutants across gradients of glucose and nitrogen concentrations and show that the preference for fermentation over respiration is influenced by the availability of glucose and nitrogen. Overall, our data show that selection for high growth rates can lead to an enhanced Crabtree phenotype by the way of beneficial mutations that permit aerobic fermentation at a greater range of glucose concentrations.
| Original language | English |
|---|---|
| Pages (from-to) | 111-117 |
| Number of pages | 7 |
| Journal | Journal of Molecular Evolution |
| Volume | 86 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Feb 2018 |
Keywords
- Beneficial mutations
- Crabtree
- Experimental evolution
- Fitness effects
- Trade-off
- Yeast
Cite this
}
Beneficial Mutations from Evolution Experiments Increase Rates of Growth and Fermentation. / Sezmis, Aysha L.; Malerba, Martino E.; Marshall, Dustin J.; McDonald, Michael J.
In: Journal of Molecular Evolution, Vol. 86, No. 2, 01.02.2018, p. 111-117.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Beneficial Mutations from Evolution Experiments Increase Rates of Growth and Fermentation
AU - Sezmis, Aysha L.
AU - Malerba, Martino E.
AU - Marshall, Dustin J.
AU - McDonald, Michael J.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - A major goal of evolutionary biology is to understand how beneficial mutations translate into increased fitness. Here, we study beneficial mutations that arise in experimental populations of yeast evolved in glucose-rich media. We find that fitness increases are caused by enhanced maximum growth rate (R) that come at the cost of reduced yield (K). We show that for some of these mutants, high R coincides with higher rates of ethanol secretion, suggesting that higher growth rates are due to an increased preference to utilize glucose through the fermentation pathway, instead of respiration. We examine the performance of mutants across gradients of glucose and nitrogen concentrations and show that the preference for fermentation over respiration is influenced by the availability of glucose and nitrogen. Overall, our data show that selection for high growth rates can lead to an enhanced Crabtree phenotype by the way of beneficial mutations that permit aerobic fermentation at a greater range of glucose concentrations.
AB - A major goal of evolutionary biology is to understand how beneficial mutations translate into increased fitness. Here, we study beneficial mutations that arise in experimental populations of yeast evolved in glucose-rich media. We find that fitness increases are caused by enhanced maximum growth rate (R) that come at the cost of reduced yield (K). We show that for some of these mutants, high R coincides with higher rates of ethanol secretion, suggesting that higher growth rates are due to an increased preference to utilize glucose through the fermentation pathway, instead of respiration. We examine the performance of mutants across gradients of glucose and nitrogen concentrations and show that the preference for fermentation over respiration is influenced by the availability of glucose and nitrogen. Overall, our data show that selection for high growth rates can lead to an enhanced Crabtree phenotype by the way of beneficial mutations that permit aerobic fermentation at a greater range of glucose concentrations.
KW - Beneficial mutations
KW - Crabtree
KW - Experimental evolution
KW - Fitness effects
KW - Trade-off
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=85040778678&partnerID=8YFLogxK
U2 - 10.1007/s00239-018-9829-9
DO - 10.1007/s00239-018-9829-9
M3 - Article
AN - SCOPUS:85040778678
VL - 86
SP - 111
EP - 117
JO - Journal of Molecular Evolution
JF - Journal of Molecular Evolution
SN - 0022-2844
IS - 2
ER -