Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma

Julien Wist; Philipp Nitschke; Ricardo Conde; Angela de Diego; Maider Bizkarguenaga; Samantha Lodge; Drew Hall; Zhonglin Chai; Wenjun Wang; Sudhir Kowlessur; Marcio Fernando Cobo; Niels Pompe; Birk Schütz; Hartmut Schäfer; Manfred Spraul; Claire Cannet; Tammo Diercks; Nieves Embade; Elaine Holmes; Oscar Millet; Jeremy K. Nicholson

doi:10.1021/acs.analchem.4c04660

Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma

Julien Wist
, Philipp Nitschke
, Ricardo Conde
, Angela de Diego
, Maider Bizkarguenaga
, Samantha Lodge
, Drew Hall
, Zhonglin Chai
, Wenjun Wang
, Sudhir Kowlessur
, Marcio Fernando Cobo
, Niels Pompe
, Birk Schütz
, Hartmut Schäfer
, Manfred Spraul
, Claire Cannet
, Tammo Diercks
, Nieves Embade
, Elaine Holmes
, Oscar Millet

Jeremy K. Nicholson

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

We report the successful development and translation of high-field nuclear magnetic resonance (NMR) based comprehensive lipoprotein analysis to routine benchtop systems. This demonstrates the potential to reimagine population level cardiovascular disease risk analysis and individual level screening based on blood sampling. Using a quantitative calibration approach, we obtained stable and reproducible results from multiple sites, despite reduced spectral dispersion and sensitivity at lower field strengths. Our study shows that 25 out of 28 major lipoprotein parameters, including key cardiometabolic risk markers, were faithfully measured using benchtop NMR systems within 15 min. This development has significant implications for making a powerful diagnostic tool widely available, enhancing the potential for longitudinal personalized medicine through molecular phenotyping in the clinic.

Original language	English
Pages (from-to)	6399-6409
Number of pages	11
Journal	Analytical Chemistry
Volume	97
Issue number	12
DOIs	https://doi.org/10.1021/acs.analchem.4c04660
Publication status	Published - 1 Apr 2025

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Wist, J., Nitschke, P., Conde, R., de Diego, A., Bizkarguenaga, M., Lodge, S., Hall, D., Chai, Z., Wang, W., Kowlessur, S., Cobo, M. F., Pompe, N., Schütz, B., Schäfer, H., Spraul, M., Cannet, C., Diercks, T., Embade, N., Holmes, E., ... Nicholson, J. K. (2025). Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma. Analytical Chemistry, 97(12), 6399-6409. https://doi.org/10.1021/acs.analchem.4c04660

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title = "Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma",

abstract = "We report the successful development and translation of high-field nuclear magnetic resonance (NMR) based comprehensive lipoprotein analysis to routine benchtop systems. This demonstrates the potential to reimagine population level cardiovascular disease risk analysis and individual level screening based on blood sampling. Using a quantitative calibration approach, we obtained stable and reproducible results from multiple sites, despite reduced spectral dispersion and sensitivity at lower field strengths. Our study shows that 25 out of 28 major lipoprotein parameters, including key cardiometabolic risk markers, were faithfully measured using benchtop NMR systems within 15 min. This development has significant implications for making a powerful diagnostic tool widely available, enhancing the potential for longitudinal personalized medicine through molecular phenotyping in the clinic.",

author = "Julien Wist and Philipp Nitschke and Ricardo Conde and \{de Diego\}, Angela and Maider Bizkarguenaga and Samantha Lodge and Drew Hall and Zhonglin Chai and Wenjun Wang and Sudhir Kowlessur and Cobo, \{Marcio Fernando\} and Niels Pompe and Birk Sch{\"u}tz and Hartmut Sch{\"a}fer and Manfred Spraul and Claire Cannet and Tammo Diercks and Nieves Embade and Elaine Holmes and Oscar Millet and Nicholson, \{Jeremy K.\}",

note = "Publisher Copyright: {\textcopyright} 2025 The Authors. Published by American Chemical Society.",

year = "2025",

month = apr,

day = "1",

doi = "10.1021/acs.analchem.4c04660",

language = "English",

volume = "97",

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Wist, J, Nitschke, P, Conde, R, de Diego, A, Bizkarguenaga, M, Lodge, S, Hall, D, Chai, Z, Wang, W, Kowlessur, S, Cobo, MF, Pompe, N, Schütz, B, Schäfer, H, Spraul, M, Cannet, C, Diercks, T, Embade, N, Holmes, E, Millet, O & Nicholson, JK 2025, 'Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma', Analytical Chemistry, vol. 97, no. 12, pp. 6399-6409. https://doi.org/10.1021/acs.analchem.4c04660

TY - JOUR

T1 - Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma

AU - Wist, Julien

AU - Nitschke, Philipp

AU - Conde, Ricardo

AU - de Diego, Angela

AU - Bizkarguenaga, Maider

AU - Lodge, Samantha

AU - Hall, Drew

AU - Chai, Zhonglin

AU - Wang, Wenjun

AU - Kowlessur, Sudhir

AU - Cobo, Marcio Fernando

AU - Pompe, Niels

AU - Schütz, Birk

AU - Schäfer, Hartmut

AU - Spraul, Manfred

AU - Cannet, Claire

AU - Diercks, Tammo

AU - Embade, Nieves

AU - Holmes, Elaine

AU - Millet, Oscar

AU - Nicholson, Jeremy K.

PY - 2025/4/1

Y1 - 2025/4/1

N2 - We report the successful development and translation of high-field nuclear magnetic resonance (NMR) based comprehensive lipoprotein analysis to routine benchtop systems. This demonstrates the potential to reimagine population level cardiovascular disease risk analysis and individual level screening based on blood sampling. Using a quantitative calibration approach, we obtained stable and reproducible results from multiple sites, despite reduced spectral dispersion and sensitivity at lower field strengths. Our study shows that 25 out of 28 major lipoprotein parameters, including key cardiometabolic risk markers, were faithfully measured using benchtop NMR systems within 15 min. This development has significant implications for making a powerful diagnostic tool widely available, enhancing the potential for longitudinal personalized medicine through molecular phenotyping in the clinic.

AB - We report the successful development and translation of high-field nuclear magnetic resonance (NMR) based comprehensive lipoprotein analysis to routine benchtop systems. This demonstrates the potential to reimagine population level cardiovascular disease risk analysis and individual level screening based on blood sampling. Using a quantitative calibration approach, we obtained stable and reproducible results from multiple sites, despite reduced spectral dispersion and sensitivity at lower field strengths. Our study shows that 25 out of 28 major lipoprotein parameters, including key cardiometabolic risk markers, were faithfully measured using benchtop NMR systems within 15 min. This development has significant implications for making a powerful diagnostic tool widely available, enhancing the potential for longitudinal personalized medicine through molecular phenotyping in the clinic.

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AN - SCOPUS:105001807633

SN - 0003-2700

VL - 97

SP - 6399

EP - 6409

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 12

ER -

Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma

Abstract

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