Behavioral assays to study oxygen and carbon dioxide sensing in Caenorhabditis elegans

Teresa Rojo Romanos, Leelee Ng, Roger Pocock

Research output: Contribution to journalArticle

Abstract

Animals use behavioral strategies to seek optimal environments. Population behavioral assays provide a robust means to determine the effect of genetic perturbations on the ability of animals to sense and respond to changes in the environment. Here, we describe a C. elegans population behavioral assay used to measure locomotory responses to changes in environmental oxygen (O2) and carbon dioxide (CO2) concentrations. These behavioral assays are high-throughput and enable examination of genetic, neuronal and circuit function.
LanguageEnglish
Article numbere2679
Number of pages14
JournalBio-protocol
Volume8
Issue number1
DOIs
StatePublished - 5 Jan 2018

Keywords

  • c. elegans
  • behavior
  • oxygen
  • carbon dioxide
  • sensing

Cite this

@article{695609b7a4434fcdbacfff291ef9f815,
title = "Behavioral assays to study oxygen and carbon dioxide sensing in Caenorhabditis elegans",
abstract = "Animals use behavioral strategies to seek optimal environments. Population behavioral assays provide a robust means to determine the effect of genetic perturbations on the ability of animals to sense and respond to changes in the environment. Here, we describe a C. elegans population behavioral assay used to measure locomotory responses to changes in environmental oxygen (O2) and carbon dioxide (CO2) concentrations. These behavioral assays are high-throughput and enable examination of genetic, neuronal and circuit function.",
keywords = "c. elegans, behavior, oxygen, carbon dioxide , sensing",
author = "Romanos, {Teresa Rojo} and Leelee Ng and Roger Pocock",
year = "2018",
month = "1",
day = "5",
doi = "10.21769/BioProtoc.2679",
language = "English",
volume = "8",
journal = "Bio-protocol",
issn = "2331-8325",
number = "1",

}

Behavioral assays to study oxygen and carbon dioxide sensing in Caenorhabditis elegans. / Romanos, Teresa Rojo; Ng, Leelee; Pocock, Roger.

In: Bio-protocol, Vol. 8, No. 1, e2679, 05.01.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Behavioral assays to study oxygen and carbon dioxide sensing in Caenorhabditis elegans

AU - Romanos,Teresa Rojo

AU - Ng,Leelee

AU - Pocock,Roger

PY - 2018/1/5

Y1 - 2018/1/5

N2 - Animals use behavioral strategies to seek optimal environments. Population behavioral assays provide a robust means to determine the effect of genetic perturbations on the ability of animals to sense and respond to changes in the environment. Here, we describe a C. elegans population behavioral assay used to measure locomotory responses to changes in environmental oxygen (O2) and carbon dioxide (CO2) concentrations. These behavioral assays are high-throughput and enable examination of genetic, neuronal and circuit function.

AB - Animals use behavioral strategies to seek optimal environments. Population behavioral assays provide a robust means to determine the effect of genetic perturbations on the ability of animals to sense and respond to changes in the environment. Here, we describe a C. elegans population behavioral assay used to measure locomotory responses to changes in environmental oxygen (O2) and carbon dioxide (CO2) concentrations. These behavioral assays are high-throughput and enable examination of genetic, neuronal and circuit function.

KW - c. elegans

KW - behavior

KW - oxygen

KW - carbon dioxide

KW - sensing

U2 - 10.21769/BioProtoc.2679

DO - 10.21769/BioProtoc.2679

M3 - Article

VL - 8

JO - Bio-protocol

T2 - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 1

M1 - e2679

ER -