Batch and dynamic adsorption of lysozyme from chicken egg white on dye-affinity nanofiber membranes modified by ethylene diamine and chitosan

Pau Loke Show, Chien Wei Ooi, Xin Jiat Lee, Chi Lin Yang, Bing Lan Liu, Yu Kaung Chang

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16 Citations (Scopus)

Abstract

Adsorption of lysozyme on the dye-affinity nanofiber membranes was investigated in batch and dynamic modes. The membrane matrix was made of electrospun polyacrylonitrile nanofibers that were grafted with ethylene diamine (EDA) and/or chitosan (CS) for the coupling of Reactive Blue 49 dye. The physicochemical properties of these dye-immobilized nanofiber membranes (P-EDA-Dye and P-CS-Dye) were characterized microscopically, spectroscopically and thermogravimetrically. The capacities of lysozyme adsorption by the dye-affinity nanofiber membranes were evaluated under various conditions, namely pH, dye immobilized density, and loading flow rate. The adsorption of lysozyme to the dye-affinity nanofiber membranes was well fitted by Langmuir isotherm and pseudo-second kinetic models. P-CS-Dye nanofiber membrane had a better performance in the dynamic adsorption of lysozyme from complex chicken egg white solution. It was observed that after five cycles of adsorption-desorption, the dye-affinity nanofiber membrane did not show a significant loss in its capacity for lysozyme adsorption. The robustness as well as high dynamic adsorption capability of P-CS-Dye nanofiber membrane are promising for the efficient recovery of lysozyme from complex feedstock via nanofiber membrane chromatography.

Original languageEnglish
Pages (from-to)1711-1724
Number of pages14
JournalInternational Journal of Biological Macromolecules
Volume162
DOIs
Publication statusPublished - 1 Nov 2020

Keywords

  • Adsorption
  • Chitosan
  • Lysozyme
  • Nanofiber membrane
  • Reactive Blue 49
  • Surface modification

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