Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens

Luisa Granziero; Peter Nelboeck; Marc Bedoucha; Antonio Lanzavecchia; Hugh H. Reid

doi:10.1016/S0022-1759(97)00018-5

Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens

Luisa Granziero, Peter Nelboeck, Marc Bedoucha, Antonio Lanzavecchia, Hugh H. Reid

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

We describe a method for the production of baculovirus-based cDNA libraries. By staining with monoclonal antibodies, single positive cells can be sorted and the virus encoding for the surface epitope can be isolated by limiting dilution. We have used this method to isolate cDNAs encoding several cell-surface antigens.

Original language	English
Pages (from-to)	131-139
Number of pages	9
Journal	Journal of Immunological Methods
Volume	203
Issue number	2
DOIs	https://doi.org/10.1016/S0022-1759(97)00018-5
Publication status	Published - 25 Apr 1997
Externally published	Yes

Keywords

baculovirus
expression cloning
surface antigen

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10.1016/S0022-1759(97)00018-5

Cite this

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title = "Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens",

abstract = "We describe a method for the production of baculovirus-based cDNA libraries. By staining with monoclonal antibodies, single positive cells can be sorted and the virus encoding for the surface epitope can be isolated by limiting dilution. We have used this method to isolate cDNAs encoding several cell-surface antigens.",

keywords = "baculovirus, expression cloning, surface antigen",

author = "Luisa Granziero and Peter Nelboeck and Marc Bedoucha and Antonio Lanzavecchia and Reid, \{Hugh H.\}",

year = "1997",

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T1 - Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens

AU - Granziero, Luisa

AU - Nelboeck, Peter

AU - Bedoucha, Marc

AU - Lanzavecchia, Antonio

AU - Reid, Hugh H.

PY - 1997/4/25

Y1 - 1997/4/25

N2 - We describe a method for the production of baculovirus-based cDNA libraries. By staining with monoclonal antibodies, single positive cells can be sorted and the virus encoding for the surface epitope can be isolated by limiting dilution. We have used this method to isolate cDNAs encoding several cell-surface antigens.

AB - We describe a method for the production of baculovirus-based cDNA libraries. By staining with monoclonal antibodies, single positive cells can be sorted and the virus encoding for the surface epitope can be isolated by limiting dilution. We have used this method to isolate cDNAs encoding several cell-surface antigens.

KW - baculovirus

KW - expression cloning

KW - surface antigen

UR - https://www.scopus.com/pages/publications/0030935169

U2 - 10.1016/S0022-1759(97)00018-5

DO - 10.1016/S0022-1759(97)00018-5

M3 - Article

C2 - 9149806

AN - SCOPUS:0030935169

SN - 0022-1759

VL - 203

SP - 131

EP - 139

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 2

ER -

Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens

Abstract

Keywords

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