Bacterial lipopolysaccharide inhibits influenza virus infection of human macrophages and the consequent induction of CD8+ T cell immunity

Kirsty R. Short, Marloes Vissers, Stan de Kleijn, Katherine Kedzierska, Emma Grant, Patrick C. Reading, Peter W.M. Hermans, Gerben Ferwerda, Dimitri A. Diavatopoulos

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14 Citations (Scopus)


It is well established that infection with influenza A virus (IAV) facilitates secondary bacterial disease. However, there is a growing body of evidence that the microbial context in which IAV infection occurs can affect both innate and adaptive responses to the virus. To date, these studies have been restricted to murine models of disease and the relevance of these findings in primary human cells remains to be elucidated. Here, we show that pre-stimulation of primary human monocyte-derived macrophages (MDMs) with the bacterial ligand lipopolysaccharide (LPS) reduces the ability of IAV to infect these cells. The inhibition of IAV infection was associated with a reduced transcription of viral RNA and the ability of LPS to induce an anti-viral/type I interferon response in human MDMs. We demonstrated that this reduced rate of viral infection is associated with a reduced ability to present a model antigen to autologous CD8+ T cells. Taken together, these data provide the first evidence that exposure to bacterial ligands like LPS can play an important role in modulating the immune response of primary human immune cells towards IAV infection, which may then have important consequences for the development of the host's adaptive immune response.

Original languageEnglish
Pages (from-to)129-139
Number of pages11
JournalJournal of Innate Immunity
Issue number2
Publication statusPublished - Feb 2014
Externally publishedYes


  • Antiviral response
  • Human macrophages
  • Influenza virus
  • Lipopolysaccharides

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