@article{2a51b14df5f04166b45e0a8d53a7ca89,
title = "Bacterial flagella hijack type IV pili proteins to control motility",
abstract = "Bacterial flagella and type IV pili (TFP) are surface appendages that enable motility and mechanosensing through distinct mechanisms. These structures were previously thought to have no components in common. Here, we report that TFP and some flagella share proteins PilO, PilN, and PilM, which we identified as part of the Helicobacter pylori flagellar motor. H. pylori mutants lacking PilO or PilN migrated better than wild type in semisolid agar because they continued swimming rather than aggregated into microcolonies, mimicking the TFP-regulated surface response. Like their TFP homologs, flagellar PilO/PilN heterodimers formed a peripheral cage that encircled the flagellar motor. These results indicate that PilO and PilN act similarly in flagella and TFP by differentially regulating motility and microcolony formation when bacteria encounter surfaces.",
keywords = "flagellar motor, mechanoresponse, nanomachine",
author = "Xiaolin Liu and Shoichi Tachiyama and Xiaotian Zhou and Mathias, {Rommel A.} and Bonny, {Sharmin Q.} and Khan, {Mohammad F.} and Yue Xin and Anna Roujeinikova and Jun Liu and Ottemann, {Karen M.}",
note = "Funding Information: ACKNOWLEDGMENTS. We would like to thank Jashwin Sagoo (UCSC) for useful discussions that framed PilO function, Dr. Nina Salama (Fred Hutchison Cancer Research Center) for providing genomic DNA for several mutant alleles, Dr. Lori Burrows (McMaster University) for helpful discussions about the alignment complex function, Camilla Faoro (Monash University) for assistance with SPR experiments, Naveen Vankadari (Monash University) for preliminary protein preparation work, Samira Heydari (Yale University) for assisting cryo-ET sample preparation and data analyses, and Jennifer Aronson (Yale University) for valuable comments on the manuscript. We thank Dr. Benjamin Abrams at UCSC Life Sciences Microscopy Center, for training and assistance using Zeiss Axio Imager (RRID: SCR_021135), and Dr. Tom Yuzvinsky at UCSC W. M. Keck Center for Nanoscale Optofluidics for assistance using the dual-beam Microscope. The described project was supported by the National Institute of Allergy and Infectious Disease (NIAID) grant R01AI164682 to K.M.O., the Australian Research Council grant DP210103056 to A.R., and a student fellowship from the China Scholarship Council (CSC) 201904910692 to X.L., S.T., and J.L. are supported by the NIAID grants R01AI087846 and R01AI132818. The funders had no role in study design, data collection, or interpretation, or the decision to submit the work for publication. Publisher Copyright: {\textcopyright} 2024 the Author(s).",
year = "2024",
doi = "10.1073/pnas.2317452121",
language = "English",
volume = "121",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "4",
}