Bacterial actin MreB forms antiparallel double filaments

Fusinita van den Ent, Thierry Izore, Tanmay AM Bharat, Christopher M Johnson, Jan Lowe

Research output: Contribution to journalArticleResearchpeer-review

73 Citations (Scopus)

Abstract

Filaments of all actin-like proteins known to date are assembled from pairs of protofilaments that are arranged in a parallel fashion, generating polarity. In this study, we show that the prokaryotic actin homologue MreB forms pairs of protofilaments that adopt an antiparallel arrangement in vitro and in vivo. We provide an atomic view of antiparallel protofilaments of Caulobacter MreB as apparent from crystal structures. We show that a protofilament doublet is essential for MreB's function in cell shape maintenance and demonstrate by in vivo site-specific cross-linking the antiparallel orientation of MreB protofilaments in E. coli. 3D cryo-EM shows that pairs of protofilaments of Caulobacter MreB tightly bind to membranes. Crystal structures of different nucleotide and polymerisation states of Caulobacter MreB reveal conserved conformational changes accompanying antiparallel filament formation. Finally, the antimicrobial agents A22/MP265 are shown to bind close to the bound nucleotide of MreB, presumably preventing nucleotide hydrolysis and destabilising double protofilaments.
Original languageEnglish
Article numbere02634
Number of pages22
JournaleLife
Volume2014
Issue number3
DOIs
Publication statusPublished - 2 May 2014
Externally publishedYes

Keywords

  • Cell shape
  • Cell wall
  • Elongasome
  • MreB
  • MreBCD

Cite this

van den Ent, F., Izore, T., Bharat, T. AM., Johnson, C. M., & Lowe, J. (2014). Bacterial actin MreB forms antiparallel double filaments. eLife, 2014(3), [e02634]. https://doi.org/10.7554/eLife.02634
van den Ent, Fusinita ; Izore, Thierry ; Bharat, Tanmay AM ; Johnson, Christopher M ; Lowe, Jan. / Bacterial actin MreB forms antiparallel double filaments. In: eLife. 2014 ; Vol. 2014, No. 3.
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van den Ent, F, Izore, T, Bharat, TAM, Johnson, CM & Lowe, J 2014, 'Bacterial actin MreB forms antiparallel double filaments', eLife, vol. 2014, no. 3, e02634. https://doi.org/10.7554/eLife.02634

Bacterial actin MreB forms antiparallel double filaments. / van den Ent, Fusinita; Izore, Thierry; Bharat, Tanmay AM; Johnson, Christopher M; Lowe, Jan.

In: eLife, Vol. 2014, No. 3, e02634, 02.05.2014.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Johnson, Christopher M

AU - Lowe, Jan

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van den Ent F, Izore T, Bharat TAM, Johnson CM, Lowe J. Bacterial actin MreB forms antiparallel double filaments. eLife. 2014 May 2;2014(3). e02634. https://doi.org/10.7554/eLife.02634