BAC-based PCR fragment microarray: High-resolution detection of chromosomal deletion and duplication breakpoints

Hua Ren, Wendy Francis, Amber Boys, Anderly C. Chueh, Nick Wong, Phung La, Lee H. Wong, Jacinta Ryan, Howard R. Slater, K. H Andy Choo

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

The introduction of molecular techniques in conjunction with classical cytogenetic methods has in recent years greatly improved the diagnostic potential for chromosomal abnormalities. In particular, microarray-comparative genomic hybridization (CGH) based on the use of BAC clones promises a sensitive strategy for the detection of DNA copy-number changes on a genomewide scale, offering a resolution as high as >30,000 "bands" (as defined by the number of BACs within the currently highest-density BAC array) [Ishkanian et al., 2004]. We have tested the possibility of further increasing this resolution using PCR fragments generated from individual BAC clones. Using this approach, we have efficiently defined the proximal and distal breakpoints in two cytogenetic cases, one duplication and one deletion, to within 5-20 kb. The results support the potential use of BAC-based PCR fragments to further improve the resolution of the microarray-CGH strategy by an order of magnitude.

Original languageEnglish
Pages (from-to)476-482
Number of pages7
JournalHuman Mutation
Volume25
Issue number5
DOIs
Publication statusPublished - May 2005
Externally publishedYes

Keywords

  • Chromosome abnormalities
  • Cytogenetics
  • Microarray-CGH
  • PCR-microarray

Cite this