Axonal accumulation of synaptic markers in APP transgenic Drosophila depends on the NPTY motif and is paralleled by defects in synaptic plasticity

Patricia Rusu, Anna Jansen, Peter Soba, Joachim Kirsch, Alexander Löwer, Gunter Merdes, Yung Hui Kuan, Anita Jung, Konrad Beyreuther, Ole Kjaerulff, Stefan Kins

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19 Citations (Scopus)


Alzheimer's disease (AD) is characterized by neurofibrillary tangles and extracellular plaques, which consist mainly of β-amyloid derived from the β-amyloid precursor protein (APP). An additional feature of AD is axonopathy, which might contribute to impairment of cognitive functions. Specifically, axonal transport defects have been reported in AD animal models, including mice and flies that overexpress APP and tau. Here we demonstrate that the APP-induced traffic jam of vesicles in peripheral nerves of Drosophila melanogaster larvae depends on the four residues NPTY motif in the APP intracellular domain. Furthermore, heterologous expression of Fe65 and JIP1b, scaffolding proteins interacting with the NPTY motif, also perturb axonal transport. Together, these data indicate that JIP1b or Fe65 may be involved in the APP-induced axonal transport defect. Moreover, we have characterized neurotransmission at the neuromuscular junction in transgenic larvae that express human APP. Consistent with the observation that these larvae do not show any obvious movement deficits, we found no changes in basal synaptic transmission. However, short-term synaptic plasticity was affected by overexpression of APP. Together, our results show that overexpression of APP induces partial stalling of axonal transport vesicles, paralleled by abnormalities in synaptic plasticity, which may provide a functional link to the deterioration of cognitive functions observed in AD.

Original languageEnglish
Pages (from-to)1079-1086
Number of pages8
JournalEuropean Journal of Neuroscience
Issue number4
Publication statusPublished - 1 Feb 2007
Externally publishedYes


  • APPL
  • Axonopathies
  • Axonopathy
  • Neurodegenerative disease
  • Neuromuscular junction

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