TY - JOUR
T1 - Average theoretical peak time as a metric to analytical speed in one dimensional and multidimensional gas chromatographic separations
AU - von Mühlen, Carin
AU - Mangelli, Luciana N.R.
AU - Marriott, Philip J.
N1 - Funding Information:
This study was financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001.
Publisher Copyright:
© 2022
PY - 2022/3/29
Y1 - 2022/3/29
N2 - The definition of a chromatographic analysis speed based simply on analysis time is an outdated concept to define conventional chromatography, fast chromatography, and emerging high-resolution techniques such as comprehensive two-dimensional and comprehensive three-dimensional gas chromatography. Here, the metric average theoretical peak time (ATPT) is proposed for separation speed, considering conventional and multidimensional separations. ATPT can be defined as the time (in ms per peak) needed to elute a theoretical peak in a chromatographic system. Using this metric, it is possible to define ranges, proposed for a normal speed (ATPT higher than 4000 ms/peak), high speed (ATPT range from 600 to 4000 ms/peak), very high speed (ATPT range from 200 to 600 ms/peak), hyper speed (ATPT range from 3.3 to 200 ms/peak) and ultra high speed chromatography (ATPT lower than 3.3 ms/peak), that combines time and efficiency metrics. This metric was applied in several contexts to demonstrate its robustness to evaluate chromatographic separations for different techniques and analytical conditions. Applications also demonstrate the advantages of the use of ATPT as a method development metric tool.
AB - The definition of a chromatographic analysis speed based simply on analysis time is an outdated concept to define conventional chromatography, fast chromatography, and emerging high-resolution techniques such as comprehensive two-dimensional and comprehensive three-dimensional gas chromatography. Here, the metric average theoretical peak time (ATPT) is proposed for separation speed, considering conventional and multidimensional separations. ATPT can be defined as the time (in ms per peak) needed to elute a theoretical peak in a chromatographic system. Using this metric, it is possible to define ranges, proposed for a normal speed (ATPT higher than 4000 ms/peak), high speed (ATPT range from 600 to 4000 ms/peak), very high speed (ATPT range from 200 to 600 ms/peak), hyper speed (ATPT range from 3.3 to 200 ms/peak) and ultra high speed chromatography (ATPT lower than 3.3 ms/peak), that combines time and efficiency metrics. This metric was applied in several contexts to demonstrate its robustness to evaluate chromatographic separations for different techniques and analytical conditions. Applications also demonstrate the advantages of the use of ATPT as a method development metric tool.
KW - ATPT
KW - Chromatographic speed
KW - Comprehensive two-dimensional gas chromatography
KW - Fast chromatography
KW - GC × GC
KW - Peak capacity
UR - http://www.scopus.com/inward/record.url?scp=85124473586&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2022.462887
DO - 10.1016/j.chroma.2022.462887
M3 - Article
AN - SCOPUS:85124473586
SN - 0021-9673
VL - 1667
JO - Journal of Chromatography A
JF - Journal of Chromatography A
M1 - 462887
ER -