Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 3725 - 3740 |
| Number of pages | 16 |
| Journal | Cellular and Molecular Life Sciences |
| Volume | 68 |
| Issue number | 22 |
| DOIs | |
| Publication status | Published - 2011 |
Cite this
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Autophagic activity in cortical neurons under acute oxidative stress directly contributes to cell death. / Higgins, Gavin C; Devenish, Rodney James; Beart, Philip M; Nagley, Phillip.
In: Cellular and Molecular Life Sciences, Vol. 68, No. 22, 2011, p. 3725 - 3740.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Autophagic activity in cortical neurons under acute oxidative stress directly contributes to cell death
AU - Higgins, Gavin C
AU - Devenish, Rodney James
AU - Beart, Philip M
AU - Nagley, Phillip
PY - 2011
Y1 - 2011
N2 - Primary neurons undergo insult-dependent programmed cell death. We examined autophagy as a process contributing to cell death in cortical neurons after treatment with either hydrogen peroxide (H(2)O(2)) or staurosporine. Although caspase-9 activation and cleavage of procaspase-3 were significant following staurosporine treatment, neither was observed following H(2)O(2) treatment, indicating a non-apoptotic death. Autophagic activity increased rapidly with H(2)O(2), but slowly with staurosporine, as quantified by processing of endogenous LC3. Autophagic induction by both stressors increased the abundance of fluorescent puncta formed by GFP-LC3, which could be blocked by 3-methyladenine. Significantly, such inhibition of autophagy blocked cell death induced by H(2)O(2) but not staurosporine. Suppression of Atg7 inhibited cell death by H(2)O(2), but not staurosporine, whereas suppression of Beclin 1 prevented cell death by both treatments, suggesting it has a complex role regulating both apoptosis and autophagy. We conclude that autophagic mechanisms are activated in an insult-dependent manner and that H(2)O(2) induces autophagic cell death.
AB - Primary neurons undergo insult-dependent programmed cell death. We examined autophagy as a process contributing to cell death in cortical neurons after treatment with either hydrogen peroxide (H(2)O(2)) or staurosporine. Although caspase-9 activation and cleavage of procaspase-3 were significant following staurosporine treatment, neither was observed following H(2)O(2) treatment, indicating a non-apoptotic death. Autophagic activity increased rapidly with H(2)O(2), but slowly with staurosporine, as quantified by processing of endogenous LC3. Autophagic induction by both stressors increased the abundance of fluorescent puncta formed by GFP-LC3, which could be blocked by 3-methyladenine. Significantly, such inhibition of autophagy blocked cell death induced by H(2)O(2) but not staurosporine. Suppression of Atg7 inhibited cell death by H(2)O(2), but not staurosporine, whereas suppression of Beclin 1 prevented cell death by both treatments, suggesting it has a complex role regulating both apoptosis and autophagy. We conclude that autophagic mechanisms are activated in an insult-dependent manner and that H(2)O(2) induces autophagic cell death.
UR - http://www.ncbi.nlm.nih.gov/pubmed/21437645
U2 - 10.1007/s00018-011-0667-9
DO - 10.1007/s00018-011-0667-9
M3 - Article
VL - 68
SP - 3725
EP - 3740
JO - Cellular and Molecular Life Sciences
JF - Cellular and Molecular Life Sciences
SN - 1420-682X
IS - 22
ER -