Projects per year
Abstract
The Caenorhabditis elegans germline is widely used as a model to study stem cell development, chromosome dynamics and apoptosis. Major readouts of germline phenotypes such as cell counting and protein expression profiling are routinely analyzed manually and in a two-dimensional manner. The major disadvantages of the existing approaches are 1) they are time-consuming and laborious and 2) there is an inability to study the effects of genetic mutations in three dimensions. Here, we demonstrate a rapid, automated method for analyzing the three-dimensional distribution of proteins, germline nuclei and cytoskeletal structures in the C. elegans germline. Using this method, we have revealed previously unappreciated germline organization and cytoskeletal structures that will have a major impact on the characterization of germline phenotypes. To conclude, our new method dramatically enhances the efficiency and resolution of C. elegans germline analysis and may be applied to other cellular structures.
Original language | English |
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Pages (from-to) | 222-228 |
Number of pages | 7 |
Journal | Developmental Biology |
Volume | 432 |
Issue number | 2 |
DOIs | |
Publication status | Published - 15 Dec 2017 |
Keywords
- Actin cytoskeleton
- Automated analysis
- Caenorhabditis elegans
- Germline
Projects
- 2 Finished
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Decoding Conserved Mechanisms That Control Neuronal Migration
National Health and Medical Research Council (NHMRC) (Australia)
1/01/16 → 31/12/18
Project: Research
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Deciphering the Function of a Schizophrenic-associated Micro-RNA
Victorian Endowment for Science, Knowledge and Innovation
1/07/15 → 30/06/18
Project: Research
Equipment
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Monash Micro Imaging
Stephen Firth (Manager), Alex Fulcher (Operator), Oleks Chernyavskiy (Operator), Margaret Rzeszutek (Other), David Potter (Manager), Volker Hilsenstein (Operator), Juan Nunez-Iglesias (Other), Stephen Cody (Manager), Irena Carmichael (Operator), Betty Kouskousis (Other), Sarah Creed (Manager) & Giulia Ballerin (Operator)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility