Automated technologies and novel techniques to accelerate protein crystallography for structural genomics

Babu A. Manjasetty, Andrew P. Turnbull, Santosh Panjikar, Konrad Büssow, Mark R. Chance

Research output: Contribution to journalReview ArticleResearchpeer-review

52 Citations (Scopus)

Abstract

The sequence infrastructure that has arisen through large-scale genomic projects dedicated to protein analysis, has provided a wealth of information and brought together scientists and institutions from all over the world. As a consequence, the development of novel technologies and methodologies in proteomics research is helping to unravel the biochemical and physiological mechanisms of complex multivariate diseases at both a functional and molecular level. In the late sixties, when X-ray crystallography had just been established, the idea of determining protein structure on an almost universal basis was akin to an impossible dream or a miracle. Yet only forty years after, automated protein structure determination platforms have been established. The widespread use of robotics in protein crystallography has had a huge impact at every stage of the pipeline from protein cloning, over-expression, purification, crystallization, data collection, structure solution, refinement, validation and data management- all of which have become more or less automated with minimal human intervention necessary. Here, recent advances in protein crystal structure analysis in the context of structural genomics will be discussed. In addition, this review aims to give an overview of recent developments in high throughput instrumentation, and technologies and strategies to accelerate protein structure/function analysis.

Original languageEnglish
Pages (from-to)612-625
Number of pages14
JournalProteomics
Volume8
Issue number4
DOIs
Publication statusPublished - Feb 2008
Externally publishedYes

Keywords

  • Protein structure analysis
  • Structural genomics
  • Structural proteomics
  • X-ray crystallography

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