Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Anqi Li, Stephen Wilson, Ian Fitzpatrick, Mehri Barabadi, Siow Teng Chan, Mirja Krause, Gina Diamanta Kusuma, David James, Rebecca Lim

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Successful commercialization of gene and cell-based therapies requires manufacturing processes that are cost-effective and scalable. Buffer exchange and product concentration are essential components for most manufacturing processes. However, at the early stages of product development, these steps are often performed manually. Manual dead-end centrifugation for buffer exchange is labor-intensive, costly, and not scalable. A closed automated system can effectively eliminate this laborious step, but implementation can be challenging. Here, we describe a newly developed cell processing device that is suitable for small- to medium-scale cell processing and aims to bridge the gap between manual processing and large-scale automation. This protocol can be easily applied to various cell types and processes by modifying the flow rate and centrifugation speed. Our protocol demonstrated high cell recovery with shorter processing times in comparison to the manual process. Cells recovered from the automated process also maintained their proliferation rates. The device can be applied as a modular component in a closed manufacturing process to accommodate steps such as buffer exchange, cell formulation, and cryopreservation.

Original languageEnglish
Number of pages9
JournalJournal of Visualized Experiments
Volume2019
Issue number154
DOIs
Publication statusPublished - 12 Dec 2019

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