TY - JOUR
T1 - Automated and ultrasensitive detection of methyl-3-quinoxaline-2-carboxylic acid by using gold nanoparticles probes SIA-rt-PCR
AU - Chen, Wei
AU - Jiang, Yuan
AU - Ji, Baoqing
AU - Zhu, Changqin
AU - Liu, Liqiang
AU - Peng, Chifang
AU - Jin, M. Kim
AU - Qiao, Ruirui
AU - Jin, Zhengyu
AU - Wang, Libing
AU - Zhu, Shuifang
AU - Xu, Chuanlai
PY - 2009/5/15
Y1 - 2009/5/15
N2 - An ultrasensitive and rapid sequential injection analysis (SIA) based on real-time PCR (SIA-rt-PCR) assay was developed by using different nanoparticles for the detection of small molecule chemicals residues. Gold (Au) nanoparticle, conjugated with goat anti-rabbit IgG and duplex strand DNA (dsDNA), was used as a substitute for chemiluminescent probes in an SIA system. By indirect competitive immunoreactions in the SIA system, the gold nanoparticles were attached to antigens which were immobilized by superparamagnetic nanoparticles (SMNPs). The dsDNA on the gold nanoparticles was dehybridized and then the single-stranded DNA (ssDNA) was collected and quantified with rt-PCR, which showed a rather low linearity range from 2.5 atto mol L-1 (aM) to 250 femto mol L-1 (fM) and the LOD was 1.4 aM. This method, which is rapid, automated and capable of high-throughput, was used to detect methyl-3-quinoxaline-2-carboxylic acid (MQCA) residues in real samples. The analytical results had a coefficient of variation of less than 15% and the recovery was 89-108%. Crown
AB - An ultrasensitive and rapid sequential injection analysis (SIA) based on real-time PCR (SIA-rt-PCR) assay was developed by using different nanoparticles for the detection of small molecule chemicals residues. Gold (Au) nanoparticle, conjugated with goat anti-rabbit IgG and duplex strand DNA (dsDNA), was used as a substitute for chemiluminescent probes in an SIA system. By indirect competitive immunoreactions in the SIA system, the gold nanoparticles were attached to antigens which were immobilized by superparamagnetic nanoparticles (SMNPs). The dsDNA on the gold nanoparticles was dehybridized and then the single-stranded DNA (ssDNA) was collected and quantified with rt-PCR, which showed a rather low linearity range from 2.5 atto mol L-1 (aM) to 250 femto mol L-1 (fM) and the LOD was 1.4 aM. This method, which is rapid, automated and capable of high-throughput, was used to detect methyl-3-quinoxaline-2-carboxylic acid (MQCA) residues in real samples. The analytical results had a coefficient of variation of less than 15% and the recovery was 89-108%. Crown
KW - Gold
KW - MQCA
KW - rt-PCR
KW - Sequential injection analysis
KW - Superparamagnetic nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=64649104414&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2009.02.015
DO - 10.1016/j.bios.2009.02.015
M3 - Article
C2 - 19303280
AN - SCOPUS:64649104414
VL - 24
SP - 2858
EP - 2863
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 9
ER -