Autologous red cell agglutination assay for HIV-1 antibodies: Simplified test with whole blood

Bruce E. Kemp, Dennis B. Rylatt, Peter G. Bundesen, Richard R. Doherty, Dale A. Mcphee, David Stapleton, Louise E. Cottis, Kim Wilson, Michele A. John, Julie M. Khan, Denise P. Dinh, Stephen Miles, Carmel J. Hillyard

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Abstract

An antibody detection procedure based on agglutination of autologous red cells has been developed for samples of whole blood. A nonagglutinating monoclonal antibody to human red blood cells conjugated to a synthetic peptide antigen (in this case residues 579 to 601 of the HIV-1 envelope precursor, Arg-Ile-Leu-Ala-Val-Glu-Arg-Tyr-Leu-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly-Cys- Ser-Gly-Lys) permitted the detection of antibodies to the human immunodeficiency virus type 1 (HIV-1) in 10 microliters of whole blood within 2 minutes. Agglutination was specifically inhibited by addition of synthetic peptide antigen but not by unrelated peptides. The frequency of false positive results was 0.1% with HIV-1 seronegative blood donors (n = 874). The false negative results were approximately 1% (n = 81). The autologous red cell agglutination test is potentially suitable for simple, rapid, qualitative screening for antibodies to a variety of antigens of medical and veterinary diagnostic significance.

Original languageEnglish
Pages (from-to)1352-1354
Number of pages3
JournalScience
Volume241
Issue number4871
DOIs
Publication statusPublished - 1 Jan 1988
Externally publishedYes

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