Aurora Kinase B, a novel regulator of TERF1 binding and telomeric integrity

Foong Lyn Chan, Benjamin Vinod, Karel Novy, Ralf B. Schittenhelm, Cheng Huang, Maheshi Udugama, Juan Nunez-Iglesias, Jane I. Lin, Linda Hii, Julie Chan, Hilda A. Pickett, Roger J. Daly, Lee H. Wong

Research output: Contribution to journalArticleResearchpeer-review

Abstract

AURKB (Aurora Kinase B) is a serine/threonine kinase better known for its role at the mitotic kinetochore during chromosome segregation. Here, we demonstrate that AURKB localizes to the telomeres in mouse embryonic stem cells, where it interacts with the essential telomere protein TERF1. Loss of AURKB function affects TERF1 telomere binding and results in aberrant telomere structure. In vitro kinase experiments successfully identified Serine 404 on TERF1 as a putative AURKB target site. Importantly, in vivo overexpression of S404-TERF1 mutants results in fragile telomere formation. These findings demonstrate that AURKB is an important regulator of telomere structural integrity.

Original languageEnglish
Pages (from-to)12340-12353
Number of pages14
JournalNucleic Acids Research
Volume45
Issue number21
DOIs
Publication statusPublished - 1 Dec 2017

Cite this

Chan, Foong Lyn ; Vinod, Benjamin ; Novy, Karel ; Schittenhelm, Ralf B. ; Huang, Cheng ; Udugama, Maheshi ; Nunez-Iglesias, Juan ; Lin, Jane I. ; Hii, Linda ; Chan, Julie ; Pickett, Hilda A. ; Daly, Roger J. ; Wong, Lee H. / Aurora Kinase B, a novel regulator of TERF1 binding and telomeric integrity. In: Nucleic Acids Research. 2017 ; Vol. 45, No. 21. pp. 12340-12353.
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abstract = "AURKB (Aurora Kinase B) is a serine/threonine kinase better known for its role at the mitotic kinetochore during chromosome segregation. Here, we demonstrate that AURKB localizes to the telomeres in mouse embryonic stem cells, where it interacts with the essential telomere protein TERF1. Loss of AURKB function affects TERF1 telomere binding and results in aberrant telomere structure. In vitro kinase experiments successfully identified Serine 404 on TERF1 as a putative AURKB target site. Importantly, in vivo overexpression of S404-TERF1 mutants results in fragile telomere formation. These findings demonstrate that AURKB is an important regulator of telomere structural integrity.",
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Aurora Kinase B, a novel regulator of TERF1 binding and telomeric integrity. / Chan, Foong Lyn; Vinod, Benjamin; Novy, Karel; Schittenhelm, Ralf B.; Huang, Cheng; Udugama, Maheshi; Nunez-Iglesias, Juan; Lin, Jane I.; Hii, Linda; Chan, Julie; Pickett, Hilda A.; Daly, Roger J.; Wong, Lee H.

In: Nucleic Acids Research, Vol. 45, No. 21, 01.12.2017, p. 12340-12353.

Research output: Contribution to journalArticleResearchpeer-review

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