Attenuating influenza a virus infection by heparin binding EGF-like growth factor

K. M. Lai, B. H. Goh, W. L. Lee

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Cell entry of influenza A virus (IAV) was reported to be promoted by epidermal growth factor receptor (EGFR). On the other hand, binding of heparin-binding EGF-like growth factor (HB-EGF) to EGFR leads to internalisation and degradation of the receptors. This study aimed to testify whether or not HB-EGF-induced downregulation of EGFR could attenuate IAV cell entry and subsequently diminish the infection. Immunoblotting and plaque assay revealed that HB-EGF-induced degradation of EGFR led to reduction of viral matrix 1 protein level and suppressed virion production. In addition, immunoblotting and imaging flow cytometric analysis demonstrated that IAV-induced phosphorylation of STAT1 and its localisation to nucleus in the early stage of infection were inhibited by HB-EGF treatment. This suggested the potential of HB-EGF in modulating uncontrolled and exaggerated inflammatory response caused by IAV infection. Together these findings attest the potential of HB-EGF mediated endocytosis and degradation of EGFR as a novel anti-IAV strategy.

Original languageEnglish
Pages (from-to)167-176
Number of pages10
JournalGrowth Factors
Volume38
Issue number3-4
DOIs
Publication statusPublished - 15 Mar 2020

Keywords

  • antiviral
  • EGFR
  • HB-EGF
  • Influenza A virus
  • STAT1
  • viral matrix 1 protein

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