ATR-Spin: An open-source 3D printed device for direct cytocentrifugation onto attenuated total reflectance crystals

David Pérez-Guaita, Zack Richardson, Guillermo Quintás, Julia Kuligowski, Diana E. Bedolla, Hugh J. Byrne, Bayden Wood

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Infrared spectroscopy (IR) enables the direct and rapid characterization of cells at the molecular level. Achieving a rapid and consistent cell preparation is critical for the development of point-of-care diagnostics for cell analysis. Here we introduce an open-source, 3D printed device for integrating the isolation, preconcentration, and measurement of attenuated total reflectance IR spectra of cells from biofluids. The tool comprises a disposable card for cytocentrifugation, equipped with magnets, which allows reproducible integration into the pathlength of the IR spectrophotometer. Preliminary results using cell culture media containing A549 cells indicate that this system enables a qualitative and quantitative characterization of cells down to 10 cells μL-1 by using a single and cost-effective device and within a few minutes.

Original languageEnglish
Pages (from-to)4743-4748
Number of pages6
JournalLab on a Chip
Volume21
Issue number24
DOIs
Publication statusPublished - 21 Dec 2021

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