@article{ceba2e273e0241fe97609147c6b47d92,
title = "ATR-Spin: An open-source 3D printed device for direct cytocentrifugation onto attenuated total reflectance crystals",
abstract = "Infrared spectroscopy (IR) enables the direct and rapid characterization of cells at the molecular level. Achieving a rapid and consistent cell preparation is critical for the development of point-of-care diagnostics for cell analysis. Here we introduce an open-source, 3D printed device for integrating the isolation, preconcentration, and measurement of attenuated total reflectance IR spectra of cells from biofluids. The tool comprises a disposable card for cytocentrifugation, equipped with magnets, which allows reproducible integration into the pathlength of the IR spectrophotometer. Preliminary results using cell culture media containing A549 cells indicate that this system enables a qualitative and quantitative characterization of cells down to 10 cells μL-1 by using a single and cost-effective device and within a few minutes.",
author = "David P{\'e}rez-Guaita and Zack Richardson and Guillermo Quint{\'a}s and Julia Kuligowski and Bedolla, {Diana E.} and Byrne, {Hugh J.} and Bayden Wood",
note = "Funding Information: We thank Kevin Chubb for assistance in the 3D printing. This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 796287. D.P.-G. Funding Information: acknowledges the financial support from the projects RPID2020-119326RA-I00 funded by MCIN/AEI/ 10.13039/ 501100011033 and RYC2019-026556-I funded by MCIN/AEI/ 10.13039/501100011033 and “ESF Investing in your future”. JK acknowledges the grant CP16/00034 from the Instituto Carlos III. JK and DPG acknowledges support from the innoviation aids from the VLC-Biomed programme (AP2020-10). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",
year = "2021",
month = dec,
day = "21",
doi = "10.1039/d1lc00813g",
language = "English",
volume = "21",
pages = "4743--4748",
journal = "Lab on a Chip",
issn = "1473-0197",
publisher = "The Royal Society of Chemistry",
number = "24",
}