Atomic force microscopy-based single virus particle spectroscopy

D. V. Korneev; A. V. Popova; V. M. Generalov; B. N. Zaitsev

doi:10.1134/S0006350916030088

Atomic force microscopy-based single virus particle spectroscopy

D. V. Korneev, A. V. Popova, V. M. Generalov, B. N. Zaitsev

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

Atomic force microscopy-based single virus particle force spectroscopy was developed using dielectrophoresis for fixing virions at the tip of an atomic force microscope (AFM) probe. Electron microscopic visualization was found to be necessary to prove the deposition of virus particles on the tip of the AFM probe, while fixation of single virions by incubating the tip with a virus suspension proved impossible. Force spectroscopy measurements were performed for the vaccinia virus, influenza virus, and bacteriophage AP22. ForceReader special software was designed for analyzing the force–distance curves.

Original language	English
Pages (from-to)	413-419
Number of pages	7
Journal	Biophysics
Volume	61
Issue number	3
DOIs	https://doi.org/10.1134/S0006350916030088
Publication status	Published - 1 May 2016
Externally published	Yes

Keywords

atomic force microscopy
dielectrophoresis
force spectroscopy
virus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1134/S0006350916030088

Cite this

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AU - Korneev, D. V.

AU - Popova, A. V.

AU - Generalov, V. M.

AU - Zaitsev, B. N.

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AB - Atomic force microscopy-based single virus particle force spectroscopy was developed using dielectrophoresis for fixing virions at the tip of an atomic force microscope (AFM) probe. Electron microscopic visualization was found to be necessary to prove the deposition of virus particles on the tip of the AFM probe, while fixation of single virions by incubating the tip with a virus suspension proved impossible. Force spectroscopy measurements were performed for the vaccinia virus, influenza virus, and bacteriophage AP22. ForceReader special software was designed for analyzing the force–distance curves.

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KW - dielectrophoresis

KW - force spectroscopy

KW - virus

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Atomic force microscopy-based single virus particle spectroscopy

Abstract

Keywords

UN SDGs

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