At least three promoters direct expression of the mouse glucocorticoid receptor gene

Uwe Strähle, Andrea Schmidt, Gavin Kelsey, A. Francis Stewart, Timothy J. Cole, Wolfgang Schmid, Günther Schütz

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132 Citations (Scopus)

Abstract

We have characterized the gene for the mouse glucocorticoid receptor. The gene spans ≈110 kilobases, and glucocorticoid receptor transcripts are assembled from nine exons. Expression of the gene is controlled by at least three promoters, resulting in glucocorticoid receptor transcripts with different 5′ nontranslated exons. One promoter is cell-specific, found to be active only in T lymphocytes. The other two promoters are active to various degrees in all cell lines and tissues so far analyzed and are located in a CpG island. The promoter activities are accompanied by DNase I hypersensitivity sites in chromatin. In contrast to a conservation of exon-intron structure, differences in promoter organization suggest a divergence between the evolution of regulatory and coding regions among members of the steroid receptor super-family. (.

Original languageEnglish
Pages (from-to)6731-6735
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number15
DOIs
Publication statusPublished - 1992
Externally publishedYes

Keywords

  • Dnase i hypersensitive sites
  • T lymphocytes

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