Associations of Th2, Th17, Treg cells, and IgA+ memory B cells with atopic disease in children: The Generation R Study

Kirsten I.M. Looman, Evelien R. van Meel, Christina Grosserichter-Wagener, Floor J.M. Vissers, Janice H. Klingenberg, Nicolette W. de Jong, Johan C. de Jongste, Suzanne G.M.A. Pasmans, Liesbeth Duijts, Menno C. van Zelm, Henriëtte A. Moll

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Abstract

Background: New insights into immune cells could contribute to treatment and monitoring of atopic disease. Because nongenetic factors shape the human immune system, we here studied these immune cells in a large cohort with atopic children with adjustment for prenatal and postnatal confounders. Methods: Information on atopic dermatitis, inhalant- and food-allergic sensitization, asthma lung function scores was obtained from 855 10-year-old children within the Generation R cohort. 11-color flow cytometry was performed to determine CD27+ and CD27IgG+, IgE+ and IgA+ memory B cells, Th1, Th2, Th17, and Treg-memory cells from venous blood. Associations between any atopic disease, the individual atopic diseases, and immune cell numbers were determined. Results: Children with any atopic disease had higher Th2, Treg, Treg-memory, and CD27+IgA+ memory B-cell numbers compared to children without atopic disease. When studying the individual diseases compared to children without the individual diseases, children with atopic dermatitis, inhalant-, and food-allergic sensitization had higher memory Treg cell numbers 12.3% (95% CI 4.2; 21.0), (11.1% (95% CI 3.0; 19.8), (23.7% (95% CI 7.9; 41.8), respectively. Children with food-allergic sensitization had higher total B and CD27+IgA+ memory B-cell numbers (15.2% [95% CI 3.2; 28.7], 22.5% [95% CI 3.9; 44.3], respectively). No associations were observed between asthma and B- or T-cell numbers. Conclusion: Children with any atopic disease and children with inhalant- and food-allergic sensitization or atopic dermatitis had higher circulating memory Treg cells, but not higher IgE+B-cell numbers. The associations of higher Treg and CD27+IgA+ B-cell numbers in children with food-allergic sensitization are suggestive of TGF-β-mediated compensation for chronic inflammation.

Original languageEnglish
Pages (from-to)178-187
Number of pages10
JournalAllergy
Volume75
Issue number1
DOIs
Publication statusPublished - 1 Jan 2020

Keywords

  • atopic disease
  • IgA memory B cell
  • IgE memory B cell
  • T helper cell
  • T regulatory cell

Cite this

Looman, K. I. M., van Meel, E. R., Grosserichter-Wagener, C., Vissers, F. J. M., Klingenberg, J. H., de Jong, N. W., de Jongste, J. C., Pasmans, S. G. M. A., Duijts, L., van Zelm, M. C., & Moll, H. A. (2020). Associations of Th2, Th17, Treg cells, and IgA+ memory B cells with atopic disease in children: The Generation R Study. Allergy, 75(1), 178-187. https://doi.org/10.1111/all.14010