Associations between DNA damage, DNA base excision repair gene variability and Alzheimer's disease risk

Dominik Kwiatkowski, Piotr Czarny, Monika Toma, Natalia Jurkowska, Agnieszka Sliwinska, Jozef Drzewoski, Agnieszka Bachurska, Janusz Szemraj, Michael Maes, Michael Berk, Kuan-Pin Su, Piotr Galecki, Tomasz Sliwinski

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

Background: Increased oxidative damage to DNA is one of the pathways involved in Alzheimer's disease (AD). Insufficient base excision repair (BER) is in part responsible for increased oxidative DNA damage. The aim of the present study was to assess the effect of polymorphic variants of BER-involved genes and the peripheral markers of DNA damage and repair in patients with AD. Material and Methods: Comet assays and TaqMan probes were used to assess DNA damage, BER efficiency and polymorphic variants of 12 BER genes in blood samples from 105 AD patients and 130 controls. The DNA repair efficacy (DRE) was calculated according to a specific equation. Results: The levels of endogenous and oxidative DNA damages were higher in AD patients than controls. The polymorphic variants of XRCC1 c.580C>T XRCC1 c.1196A>G and OGG1 c.977C>G are associated with increased DNA damage in AD. Conclusion: Our results show that oxidative stress and disturbances in DRE are particularly responsible for the elevated DNA lesions in AD. The results suggest that oxidative stress and disruption in DNA repair may contribute to increased DNA damage in AD patients and risk of this disease. In addition, disturbances in DRE may be associated with polymorphisms of OGG1 and XRCC1.

Original languageEnglish
Pages (from-to)152-171
Number of pages20
JournalDementia and Geriatric Cognitive Disorders
Volume41
Issue number3-4
DOIs
Publication statusPublished - May 2016
Externally publishedYes

Keywords

  • Alzheimer's disease risk
  • Dementia
  • DNA base excision repair
  • DNA damage
  • Oxidative stress

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