Aumento del daño en el ADN espermático en varones mayores de 40 años

Fabrizzio Horta; Marcia Madariaga; Alejandra García; Steffen Hartel; Rosita Smith

doi:10.4067/S0034-98872011000300004

Aumento del daño en el ADN espermático en varones mayores de 40 años

Translated title of the contribution: Association of age with sperm DNA fragmentation

Fabrizzio Horta, Marcia Madariaga, Alejandra García, Steffen Hartel, Rosita Smith

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

Background: There is an association between aging and an increased number of sperms with alterations in nuclear DNA. Aim: To study the association between age and fragmentation of sperm DNA. Material and Methods: Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TUNEL (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersion test) assays. Results: Compared with their younger counterparts, patients aged more than 40 years had a higher proportion of sperms with DNA fragmentation by TUNEL (20 ± 1.3 and 24 ± 1.9% respectively, p < 0.05) and SCD (22 ± 1.4 and 26 ± 1.6% respectively, p < 0.05). The results of both assays had a correlation coefficient of 0.8. No differences between groups were observed for other seminal parameters. Conclusions: Sperm DNA fragmentation increases with age in males.

Translated title of the contribution	Association of age with sperm DNA fragmentation
Original language	Spanish
Pages (from-to)	306-312
Number of pages	7
Journal	Revista Medica de Chile
Volume	139
Issue number	3
DOIs	https://doi.org/10.4067/S0034-98872011000300004
Publication status	Published - 1 Mar 2011
Externally published	Yes

Keywords

Aging
DNA fragmentation
Spermatozoa

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10.4067/S0034-98872011000300004

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title = "Aumento del da{\~n}o en el ADN esperm{\'a}tico en varones mayores de 40 a{\~n}os",

abstract = "Background: There is an association between aging and an increased number of sperms with alterations in nuclear DNA. Aim: To study the association between age and fragmentation of sperm DNA. Material and Methods: Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TUNEL (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersion test) assays. Results: Compared with their younger counterparts, patients aged more than 40 years had a higher proportion of sperms with DNA fragmentation by TUNEL (20 ± 1.3 and 24 ± 1.9% respectively, p < 0.05) and SCD (22 ± 1.4 and 26 ± 1.6% respectively, p < 0.05). The results of both assays had a correlation coefficient of 0.8. No differences between groups were observed for other seminal parameters. Conclusions: Sperm DNA fragmentation increases with age in males.",

keywords = "Aging, DNA fragmentation, Spermatozoa",

author = "Fabrizzio Horta and Marcia Madariaga and Alejandra Garc{\'i}a and Steffen Hartel and Rosita Smith",

year = "2011",

month = mar,

day = "1",

doi = "10.4067/S0034-98872011000300004",

language = "Spanish",

volume = "139",

pages = "306--312",

journal = "Revista Medica de Chile",

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T1 - Aumento del daño en el ADN espermático en varones mayores de 40 años

AU - Horta, Fabrizzio

AU - Madariaga, Marcia

AU - García, Alejandra

AU - Hartel, Steffen

AU - Smith, Rosita

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Background: There is an association between aging and an increased number of sperms with alterations in nuclear DNA. Aim: To study the association between age and fragmentation of sperm DNA. Material and Methods: Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TUNEL (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersion test) assays. Results: Compared with their younger counterparts, patients aged more than 40 years had a higher proportion of sperms with DNA fragmentation by TUNEL (20 ± 1.3 and 24 ± 1.9% respectively, p < 0.05) and SCD (22 ± 1.4 and 26 ± 1.6% respectively, p < 0.05). The results of both assays had a correlation coefficient of 0.8. No differences between groups were observed for other seminal parameters. Conclusions: Sperm DNA fragmentation increases with age in males.

AB - Background: There is an association between aging and an increased number of sperms with alterations in nuclear DNA. Aim: To study the association between age and fragmentation of sperm DNA. Material and Methods: Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TUNEL (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersion test) assays. Results: Compared with their younger counterparts, patients aged more than 40 years had a higher proportion of sperms with DNA fragmentation by TUNEL (20 ± 1.3 and 24 ± 1.9% respectively, p < 0.05) and SCD (22 ± 1.4 and 26 ± 1.6% respectively, p < 0.05). The results of both assays had a correlation coefficient of 0.8. No differences between groups were observed for other seminal parameters. Conclusions: Sperm DNA fragmentation increases with age in males.

KW - Aging

KW - DNA fragmentation

KW - Spermatozoa

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DO - 10.4067/S0034-98872011000300004

M3 - Article

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AN - SCOPUS:79960816844

VL - 139

SP - 306

EP - 312

JO - Revista Medica de Chile

JF - Revista Medica de Chile

SN - 0034-9887

IS - 3

ER -

Aumento del daño en el ADN espermático en varones mayores de 40 años

Abstract

Keywords

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