Aumento del daño en el ADN espermático en varones mayores de 40 años

Translated title of the contribution: Association of age with sperm DNA fragmentation

Fabrizzio Horta, Marcia Madariaga, Alejandra García, Steffen Hartel, Rosita Smith

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4 Citations (Scopus)


Background: There is an association between aging and an increased number of sperms with alterations in nuclear DNA. Aim: To study the association between age and fragmentation of sperm DNA. Material and Methods: Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TUNEL (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersion test) assays. Results: Compared with their younger counterparts, patients aged more than 40 years had a higher proportion of sperms with DNA fragmentation by TUNEL (20 ± 1.3 and 24 ± 1.9% respectively, p < 0.05) and SCD (22 ± 1.4 and 26 ± 1.6% respectively, p < 0.05). The results of both assays had a correlation coefficient of 0.8. No differences between groups were observed for other seminal parameters. Conclusions: Sperm DNA fragmentation increases with age in males.

Translated title of the contributionAssociation of age with sperm DNA fragmentation
Original languageSpanish
Pages (from-to)306-312
Number of pages7
JournalRevista Medica de Chile
Issue number3
Publication statusPublished - 1 Mar 2011
Externally publishedYes


  • Aging
  • DNA fragmentation
  • Spermatozoa

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