TY - JOUR
T1 - Association between polymorphisms in human tumor necrosis factor-alpha (-308) and -beta (252) genes and development of gestational diabetes mellitus
AU - Montazeri, Shabnam
AU - Nalliah, Sivalingam
AU - Radhakrishnan, Ammu Kutty
N1 - Funding Information:
The authors would like to thank Dr. Ravindran Jegasothy, Dr. Krishna Kumar, medical officers, nurses and all staff of the Department of Obstetrics and Gynecology of Hospital Tuanku Jaafar, Seremban, Malaysia for facilitating the recruitment of patients and use of clinical data. This study was supported by a Grant from the International Medical University ( IMU 125/2006 ).
PY - 2010/5
Y1 - 2010/5
N2 - Objective: The aim of this study is to investigate if an association exists between single nucleotide polymorphism (SNP) in the tumor necrosis factor-alpha (TNF-α) and TNF-β genes. Methods: The DNA was extracted and SNP in the human TNF-α and TNF-β genes at positions -308 (G/A) and 252 (A/G), respectively, was analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Plasma levels of TNF-α in different stages of pregnancy were quantified using enzyme linked immunosorbent assay (ELISA). Results: There was no significant difference in genotype and allele frequency of SNP at position -308 (G/A) in the promoter region of the human TNF-α gene as well as the SNP at position 252 (A/G) in the human TNF-β gene between the GDM and control subjects. Using the logistic regression model, it was found that the SNP in the TNF-α as well as TNF-β were not associated with development of GDM. In addition, the TNF-α levels in the plasma of GDM and control mothers were not significantly different. Conclusions: In the population studied, the SNP in position -308 (G/A) of the human TNF-α or in position 252 (A/G) of the human TNF-β gene is not an independent risk factor or a predictor for GDM.
AB - Objective: The aim of this study is to investigate if an association exists between single nucleotide polymorphism (SNP) in the tumor necrosis factor-alpha (TNF-α) and TNF-β genes. Methods: The DNA was extracted and SNP in the human TNF-α and TNF-β genes at positions -308 (G/A) and 252 (A/G), respectively, was analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Plasma levels of TNF-α in different stages of pregnancy were quantified using enzyme linked immunosorbent assay (ELISA). Results: There was no significant difference in genotype and allele frequency of SNP at position -308 (G/A) in the promoter region of the human TNF-α gene as well as the SNP at position 252 (A/G) in the human TNF-β gene between the GDM and control subjects. Using the logistic regression model, it was found that the SNP in the TNF-α as well as TNF-β were not associated with development of GDM. In addition, the TNF-α levels in the plasma of GDM and control mothers were not significantly different. Conclusions: In the population studied, the SNP in position -308 (G/A) of the human TNF-α or in position 252 (A/G) of the human TNF-β gene is not an independent risk factor or a predictor for GDM.
KW - Cytokine
KW - Gestational diabetes mellitus (GDM)
KW - Single nucleotide polymorphism
KW - TNF-α
KW - TNF-β
UR - http://www.scopus.com/inward/record.url?scp=77952884174&partnerID=8YFLogxK
U2 - 10.1016/j.diabres.2010.01.028
DO - 10.1016/j.diabres.2010.01.028
M3 - Article
C2 - 20189261
AN - SCOPUS:77952884174
SN - 0168-8227
VL - 88
SP - 139
EP - 145
JO - Diabetes Research and Clinical Practice
JF - Diabetes Research and Clinical Practice
IS - 2
ER -
