Assisted Reproductive Technologies

The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next

Richard David William Kelly, Arsalan Mahmud, Justin Charles St John

    Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

    Abstract

    Mammalian cells contain multiple identical copies of mitochondrial DNA (mtDNA) that encode genes involved in the production of ATP through the process of oxidative phosphorylation (OXPHOS). Mutations and deletions to mtDNA produce novel sequence variants, resulting in heteroplasmic mixing of mutant and wild-type molecules, which may culminate in a variety of severely debilitating and lethal multi-systemic diseases. The maternal inheritance of mtDNA is a strictly regulated process and presents a complex reproductive situation, as there are currently no proven clinical strategies available to prevent the transmission of mutant mtDNA from the mother to her offspring and to subsequent generations. Furthermore, the segregation of mtDNA during development randomly alters the mutant loading within embryonic tissues, limiting the possibility to safely predict the probability of disease manifestation. Despite these limitations, a patient may undergo an assisted reproductive program, consisting of genetic counseling and tissue sampling for biochemical and genetic screening. Encouraging studies in non-human models have developed micromanipulation approaches to reduce the transmission of mutant mtDNA between generations. However, these methodologies require further experimental validation to determine whether assisted reproductive technologies can prevent the transmission of mutant mtDNA.

    Original languageEnglish
    Title of host publicationMitochondrial DNA, Mitochondria, Disease and Stem Cells
    EditorsJustin Charles St John
    Place of PublicationUnited States
    PublisherHumana Press
    Pages157-183
    Number of pages27
    ISBN (Electronic)9781627031011
    ISBN (Print)9781627031004
    DOIs
    Publication statusPublished - 1 Jan 2013

    Cite this

    Kelly, R. D. W., Mahmud, A., & St John, J. C. (2013). Assisted Reproductive Technologies: The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next. In J. C. St John (Ed.), Mitochondrial DNA, Mitochondria, Disease and Stem Cells (pp. 157-183). United States: Humana Press. https://doi.org/10.1007/978-1-62703-101-1_7
    Kelly, Richard David William ; Mahmud, Arsalan ; St John, Justin Charles. / Assisted Reproductive Technologies : The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next. Mitochondrial DNA, Mitochondria, Disease and Stem Cells. editor / Justin Charles St John. United States : Humana Press, 2013. pp. 157-183
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    abstract = "Mammalian cells contain multiple identical copies of mitochondrial DNA (mtDNA) that encode genes involved in the production of ATP through the process of oxidative phosphorylation (OXPHOS). Mutations and deletions to mtDNA produce novel sequence variants, resulting in heteroplasmic mixing of mutant and wild-type molecules, which may culminate in a variety of severely debilitating and lethal multi-systemic diseases. The maternal inheritance of mtDNA is a strictly regulated process and presents a complex reproductive situation, as there are currently no proven clinical strategies available to prevent the transmission of mutant mtDNA from the mother to her offspring and to subsequent generations. Furthermore, the segregation of mtDNA during development randomly alters the mutant loading within embryonic tissues, limiting the possibility to safely predict the probability of disease manifestation. Despite these limitations, a patient may undergo an assisted reproductive program, consisting of genetic counseling and tissue sampling for biochemical and genetic screening. Encouraging studies in non-human models have developed micromanipulation approaches to reduce the transmission of mutant mtDNA between generations. However, these methodologies require further experimental validation to determine whether assisted reproductive technologies can prevent the transmission of mutant mtDNA.",
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    Kelly, RDW, Mahmud, A & St John, JC 2013, Assisted Reproductive Technologies: The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next. in JC St John (ed.), Mitochondrial DNA, Mitochondria, Disease and Stem Cells. Humana Press, United States, pp. 157-183. https://doi.org/10.1007/978-1-62703-101-1_7

    Assisted Reproductive Technologies : The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next. / Kelly, Richard David William; Mahmud, Arsalan; St John, Justin Charles.

    Mitochondrial DNA, Mitochondria, Disease and Stem Cells. ed. / Justin Charles St John. United States : Humana Press, 2013. p. 157-183.

    Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

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    N2 - Mammalian cells contain multiple identical copies of mitochondrial DNA (mtDNA) that encode genes involved in the production of ATP through the process of oxidative phosphorylation (OXPHOS). Mutations and deletions to mtDNA produce novel sequence variants, resulting in heteroplasmic mixing of mutant and wild-type molecules, which may culminate in a variety of severely debilitating and lethal multi-systemic diseases. The maternal inheritance of mtDNA is a strictly regulated process and presents a complex reproductive situation, as there are currently no proven clinical strategies available to prevent the transmission of mutant mtDNA from the mother to her offspring and to subsequent generations. Furthermore, the segregation of mtDNA during development randomly alters the mutant loading within embryonic tissues, limiting the possibility to safely predict the probability of disease manifestation. Despite these limitations, a patient may undergo an assisted reproductive program, consisting of genetic counseling and tissue sampling for biochemical and genetic screening. Encouraging studies in non-human models have developed micromanipulation approaches to reduce the transmission of mutant mtDNA between generations. However, these methodologies require further experimental validation to determine whether assisted reproductive technologies can prevent the transmission of mutant mtDNA.

    AB - Mammalian cells contain multiple identical copies of mitochondrial DNA (mtDNA) that encode genes involved in the production of ATP through the process of oxidative phosphorylation (OXPHOS). Mutations and deletions to mtDNA produce novel sequence variants, resulting in heteroplasmic mixing of mutant and wild-type molecules, which may culminate in a variety of severely debilitating and lethal multi-systemic diseases. The maternal inheritance of mtDNA is a strictly regulated process and presents a complex reproductive situation, as there are currently no proven clinical strategies available to prevent the transmission of mutant mtDNA from the mother to her offspring and to subsequent generations. Furthermore, the segregation of mtDNA during development randomly alters the mutant loading within embryonic tissues, limiting the possibility to safely predict the probability of disease manifestation. Despite these limitations, a patient may undergo an assisted reproductive program, consisting of genetic counseling and tissue sampling for biochemical and genetic screening. Encouraging studies in non-human models have developed micromanipulation approaches to reduce the transmission of mutant mtDNA between generations. However, these methodologies require further experimental validation to determine whether assisted reproductive technologies can prevent the transmission of mutant mtDNA.

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    Kelly RDW, Mahmud A, St John JC. Assisted Reproductive Technologies: The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next. In St John JC, editor, Mitochondrial DNA, Mitochondria, Disease and Stem Cells. United States: Humana Press. 2013. p. 157-183 https://doi.org/10.1007/978-1-62703-101-1_7