Assessment of respiratory physiology of C57BL/6 mice following Bleomycin administration using barometric plethysmography

Phillipa Milton, Hayley Dickinson, Graham Jenkin, Rebecca Lim

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

Background: Assessment of deterioration of lung function in animal models of respiratory disease traditionally relies upon quantitating biochemical markers. Plethysmography is a technique for measuring lung function that includes invasive and non-invasive methodologies. Objectives: This study used whole-body barometric plethysmography to characterize change(s) in respiratory physiology of C57BL/6 mice following bleomycin administration. Methods: Cohorts of animals were culled at 3, 7, 14 and 28 days to semi-quantitatively score the lung for fibrosis, and quantitate levels of hydroxyproline in the lung. We have described in detail the response of C57BL/6 mice to bleomycin. Results: Bleomycin-treated mice had reduced minute volume (p <0.05) and an increased total breathing cycle time (p <0.0001), which consisted of a shortened inspiration time (p <0.01) and an extended expiration time (p <0.0001). Conclusions: We have demonstrated that plethysmography can be a primary indicator of the development of respiratory disease in the mouse and would thus be suitable in assessing potential therapies since any truly effective treatment should elicit restoration of respiratory parameters in addition to improving traditional biochemical and histological indices of lung function.
Original languageEnglish
Pages (from-to)253 - 266
Number of pages14
JournalRespiration
Volume83
Issue number3
DOIs
Publication statusPublished - 2012

Cite this

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title = "Assessment of respiratory physiology of C57BL/6 mice following Bleomycin administration using barometric plethysmography",
abstract = "Background: Assessment of deterioration of lung function in animal models of respiratory disease traditionally relies upon quantitating biochemical markers. Plethysmography is a technique for measuring lung function that includes invasive and non-invasive methodologies. Objectives: This study used whole-body barometric plethysmography to characterize change(s) in respiratory physiology of C57BL/6 mice following bleomycin administration. Methods: Cohorts of animals were culled at 3, 7, 14 and 28 days to semi-quantitatively score the lung for fibrosis, and quantitate levels of hydroxyproline in the lung. We have described in detail the response of C57BL/6 mice to bleomycin. Results: Bleomycin-treated mice had reduced minute volume (p <0.05) and an increased total breathing cycle time (p <0.0001), which consisted of a shortened inspiration time (p <0.01) and an extended expiration time (p <0.0001). Conclusions: We have demonstrated that plethysmography can be a primary indicator of the development of respiratory disease in the mouse and would thus be suitable in assessing potential therapies since any truly effective treatment should elicit restoration of respiratory parameters in addition to improving traditional biochemical and histological indices of lung function.",
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Assessment of respiratory physiology of C57BL/6 mice following Bleomycin administration using barometric plethysmography. / Milton, Phillipa; Dickinson, Hayley; Jenkin, Graham; Lim, Rebecca.

In: Respiration, Vol. 83, No. 3, 2012, p. 253 - 266.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Assessment of respiratory physiology of C57BL/6 mice following Bleomycin administration using barometric plethysmography

AU - Milton, Phillipa

AU - Dickinson, Hayley

AU - Jenkin, Graham

AU - Lim, Rebecca

PY - 2012

Y1 - 2012

N2 - Background: Assessment of deterioration of lung function in animal models of respiratory disease traditionally relies upon quantitating biochemical markers. Plethysmography is a technique for measuring lung function that includes invasive and non-invasive methodologies. Objectives: This study used whole-body barometric plethysmography to characterize change(s) in respiratory physiology of C57BL/6 mice following bleomycin administration. Methods: Cohorts of animals were culled at 3, 7, 14 and 28 days to semi-quantitatively score the lung for fibrosis, and quantitate levels of hydroxyproline in the lung. We have described in detail the response of C57BL/6 mice to bleomycin. Results: Bleomycin-treated mice had reduced minute volume (p <0.05) and an increased total breathing cycle time (p <0.0001), which consisted of a shortened inspiration time (p <0.01) and an extended expiration time (p <0.0001). Conclusions: We have demonstrated that plethysmography can be a primary indicator of the development of respiratory disease in the mouse and would thus be suitable in assessing potential therapies since any truly effective treatment should elicit restoration of respiratory parameters in addition to improving traditional biochemical and histological indices of lung function.

AB - Background: Assessment of deterioration of lung function in animal models of respiratory disease traditionally relies upon quantitating biochemical markers. Plethysmography is a technique for measuring lung function that includes invasive and non-invasive methodologies. Objectives: This study used whole-body barometric plethysmography to characterize change(s) in respiratory physiology of C57BL/6 mice following bleomycin administration. Methods: Cohorts of animals were culled at 3, 7, 14 and 28 days to semi-quantitatively score the lung for fibrosis, and quantitate levels of hydroxyproline in the lung. We have described in detail the response of C57BL/6 mice to bleomycin. Results: Bleomycin-treated mice had reduced minute volume (p <0.05) and an increased total breathing cycle time (p <0.0001), which consisted of a shortened inspiration time (p <0.01) and an extended expiration time (p <0.0001). Conclusions: We have demonstrated that plethysmography can be a primary indicator of the development of respiratory disease in the mouse and would thus be suitable in assessing potential therapies since any truly effective treatment should elicit restoration of respiratory parameters in addition to improving traditional biochemical and histological indices of lung function.

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