Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction

Cyril Seillet; Gabrielle T. Belz

doi:10.1007/978-1-4939-9145-7_14

Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction

Cyril Seillet
, Gabrielle T. Belz

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

3 Citations (Scopus)

Abstract

Retroviral transduction is commonly used to modulate gene expression and is a powerful approach to understand the role of a gene using gain- or loss-of-function strategies. Retroviral vectors can stably integrate non-viral genes into host genomes, providing long-term modulation of gene expression in infected cells and their progeny. Here we describe the generation of retroviral supernatants and the steps to efficiently transduce genes in innate lymphoid cell (ILC) progenitors for subsequent analysis of ILC populations in vivo.

Original language	English
Title of host publication	Target Identification and Validation in Drug Discovery
Subtitle of host publication	Methods and Protocols
Editors	Jürgen Moll, Sebastian Carotta
Place of Publication	New York NY USA
Publisher	Humana Press
Chapter	14
Pages	231-240
Number of pages	10
Edition	2nd
ISBN (Electronic)	9781493991457
ISBN (Print)	9781493991440
DOIs	https://doi.org/10.1007/978-1-4939-9145-7_14
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1953
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Innate lymphoid cells
Lymphoid differentiation
NK cells
Transcriptional regulation

Access to Document

10.1007/978-1-4939-9145-7_14

Cite this

Seillet, C., & Belz, G. T. (2019). Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction. In J. Moll, & S. Carotta (Eds.), Target Identification and Validation in Drug Discovery: Methods and Protocols (2nd ed., pp. 231-240). (Methods in Molecular Biology; Vol. 1953). Humana Press. https://doi.org/10.1007/978-1-4939-9145-7_14

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title = "Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction",

abstract = "Retroviral transduction is commonly used to modulate gene expression and is a powerful approach to understand the role of a gene using gain- or loss-of-function strategies. Retroviral vectors can stably integrate non-viral genes into host genomes, providing long-term modulation of gene expression in infected cells and their progeny. Here we describe the generation of retroviral supernatants and the steps to efficiently transduce genes in innate lymphoid cell (ILC) progenitors for subsequent analysis of ILC populations in vivo.",

keywords = "Innate lymphoid cells, Lymphoid differentiation, NK cells, Transcriptional regulation",

author = "Cyril Seillet and Belz, \{Gabrielle T.\}",

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language = "English",

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Seillet, C & Belz, GT 2019, Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction. in J Moll & S Carotta (eds), Target Identification and Validation in Drug Discovery: Methods and Protocols. 2nd edn, Methods in Molecular Biology, vol. 1953, Humana Press, New York NY USA, pp. 231-240. https://doi.org/10.1007/978-1-4939-9145-7_14

Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction. / Seillet, Cyril; Belz, Gabrielle T.
Target Identification and Validation in Drug Discovery: Methods and Protocols. ed. / Jürgen Moll; Sebastian Carotta. 2nd. ed. New York NY USA: Humana Press, 2019. p. 231-240 (Methods in Molecular Biology; Vol. 1953).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

TY - CHAP

T1 - Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction

AU - Seillet, Cyril

AU - Belz, Gabrielle T.

PY - 2019

Y1 - 2019

N2 - Retroviral transduction is commonly used to modulate gene expression and is a powerful approach to understand the role of a gene using gain- or loss-of-function strategies. Retroviral vectors can stably integrate non-viral genes into host genomes, providing long-term modulation of gene expression in infected cells and their progeny. Here we describe the generation of retroviral supernatants and the steps to efficiently transduce genes in innate lymphoid cell (ILC) progenitors for subsequent analysis of ILC populations in vivo.

AB - Retroviral transduction is commonly used to modulate gene expression and is a powerful approach to understand the role of a gene using gain- or loss-of-function strategies. Retroviral vectors can stably integrate non-viral genes into host genomes, providing long-term modulation of gene expression in infected cells and their progeny. Here we describe the generation of retroviral supernatants and the steps to efficiently transduce genes in innate lymphoid cell (ILC) progenitors for subsequent analysis of ILC populations in vivo.

KW - Innate lymphoid cells

KW - Lymphoid differentiation

KW - NK cells

KW - Transcriptional regulation

UR - https://www.scopus.com/pages/publications/85063711671

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DO - 10.1007/978-1-4939-9145-7_14

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C2 - 30912025

AN - SCOPUS:85063711671

SN - 9781493991440

T3 - Methods in Molecular Biology

SP - 231

EP - 240

BT - Target Identification and Validation in Drug Discovery

A2 - Moll, Jürgen

A2 - Carotta, Sebastian

PB - Humana Press

CY - New York NY USA

ER -

Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction

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