Assessment of dispersive liquid-liquid microextraction conditions for gas chromatography time-of-flight mass spectrometry identification of organic compounds in honey

M. Moniruzzaman, I. Rodríguez, T. Rodríguez-Cabo, R. Cela, S. A. Sulaiman, S. H. Gan

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

The suitability of the dispersive liquid-liquid microextraction (DLLME) technique for gas chromatography (GC) characterization of minor organic compounds in honey samples is evaluated. Under optimized conditions, samples were pre-treated by liquid-liquid extraction with acetonitrile followed by DLLME using carbon tetrachloride (CCl4, 0.075mL) as extractant. The yielded settled phase was analyzed by GC using high resolution time-of-flight (TOF) mass spectrometry (MS). The whole sample preparation process is completed in approximately 10min, with a total consumption of organic solvents below 4mL, relative standard deviations lower than 12% and with more than 70 organic compounds, displaying linear retention index in the range from 990 to 2900, identified in the obtained extracts. In comparison with HS SPME extraction, higher peak intensities were attained for most volatile and semi-volatile compounds amenable to both extraction techniques. Furthermore, other species such as highly polar and water soluble benzene acids, long chain fatty acids, esters and flavonoids, which are difficult to concentrate by HS SPME, could be identified in DLLME extracts. Some of the compounds identified in DLLME extracts have been proposed as useful for samples classification and/or they are recognized as markers of honeys from certain geographic areas.

Original languageEnglish
Pages (from-to)26-36
Number of pages11
JournalJournal of Chromatography A
Volume1368
DOIs
Publication statusPublished - 14 Nov 2014
Externally publishedYes

Keywords

  • Dispersive liquid-liquid microextraction
  • Gas chromatography time-of-flight mass spectrometry
  • Honey organic compounds identification

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