Assessing the off-target effects of miRNA inhibitors on innate immune toll-like receptors

Genevieve Pepin, Jonathan Ferrand, Michael P Gantier

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

7 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are involved in most cellular processes and are deregulated in several diseases. Antisense miRNA oligonucleotides (AMOs) therefore present novel therapeutic opportunities. Currently, in vivo delivery of AMOs often relies on high doses of nucleic acids, with nonspecific uptake by most tissues. Critically, AMOs accumulate in phagocytic cells where they can interfere with immune functions, such as the activation of Toll-Like Receptors (TLRs). In this chapter, we describe a method to assess the possible off-target effects of AMOs on TLR7, 8, and 9 sensing.

Original languageEnglish
Title of host publicationDrug Target miRNA
Subtitle of host publicationMethods and Protocols
EditorsMarco F. Schmidt
Place of PublicationNew York NY USA
PublisherHumana Press
Pages127-135
Number of pages9
ISBN (Print)9781493965618
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology: Springer Protocols
Volume1517
ISSN (Print)10643745

Keywords

  • AMO
  • MicroRNA
  • TLR7
  • TLR8
  • TLR9
  • Toll-like receptors

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